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1.14.12.11: toluene dioxygenase

This is an abbreviated version!
For detailed information about toluene dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.11

Reaction

Toluene
+
NADH
 +
H+
 +
O2
=
(1S,2R)-3-Methylcyclohexa-3,5-diene-1,2-diol
 +
NAD+

Synonyms

ISPTOD, ISPTOL, More, oxygenase, toluene 2,3-di-, oxygenaseTOL, TDO, Tod, todC1C2BA, toluene 2,3-dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.11 toluene dioxygenase

Purification

Purification on EC 1.14.12.11 - toluene dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
beta-subunit of oxygenase component
-
ferredoxinTOL component of the toluene dioxygenase
-
NADH-ferredoxinTOL reductase component of toluene dioxygenase
-
oxygenase component, ISPTOL
-
Q-Sepharose FF column chromatography
-
rapid purification of oxygenase component from a polyol-responsive monoclonal antibody
-
recombinant alpha-subunit of the oxygenase component
-
recombinant TDO-F, TDO-R, TDO-O and apo-TDO-O from Escherichia coli
-