1.14.12.13: 2-halobenzoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about 2-halobenzoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.13

Reaction

a 2-halobenzoate +

NADH
+
H+
+
O2
=
catechol
+ a halide anion +
NAD+
+
CO2

Synonyms

(chloro)benzoate dioxygenase, 2-chlorobenzoate 1,2-dioxygenase, 2-halobenzoate 1,2-dioxygenase, 2-halobenzoate dioxygenase, 2-halobenzoate dioxygenase system, BenA, CbdAB, cbdABC

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.13 2-halobenzoate 1,2-dioxygenase

Cloned

Cloned on EC 1.14.12.13 - 2-halobenzoate 1,2-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains
-
expressed in Escherichia coli DH5alpha cells
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expression in Cupriavidus necator strain H850
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several constructs with relevant genes are expressed in Escherichia coli DH5alpha and S17-1 lambdapir
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several gene constructs are expressed in Escherichia coli DH5alpha, S17-1, S17-lambdapir and HMS174(DE3)
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