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1.14.12.26: chlorobenzene dioxygenase

This is an abbreviated version!
For detailed information about chlorobenzene dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.26

Reaction

chlorobenzene
+
NADH
 +
H+
 +
O2
=
(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol
 +
NAD+

Synonyms

CDO, TecA, tetrachlorobenzene dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.26 chlorobenzene dioxygenase

Cloned

Cloned on EC 1.14.12.26 - chlorobenzene dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chlorobenzene dioxygenase genes tcbAa, tcbAb, tcbAc, and tcbAd are expressed in Escherichia coli JM101 (pTEZ30) under the control of the alk regulatory system of Pseudomonas oleovorans GPo1
Q52383; Q52384; Q52385; Q52386
cloned under the strict control of the Palk promoter of Pseudomonas putida GPo1. Escherichia coli JM101 cells carrying the resulting plasmid pTEZ30 are used for the biotransformation of benzonitrile in a 2-l stirred tank bioreactor
Q52383; Q52384; Q52385; Q52386
Escherichia coli cells producing recombinant TecA enzyme dioxygenate and simultaneously eliminate a halogen substituent from 1,2,4,5-tetrachlorobenzene but show no activity toward benzene
-
expressed in Escherichia coli
-
the tcbA and tcbB genes for chlorobenzene dioxygenase and dehydrogenase are located on a transposable element, Tn5280. Tn5280 shows the features of a composite bacterial transposon with iso-insertion elements (IS1066 and IS1067) at each end of the transposon oriented in an inverted position. When a 12-kb Hindlll fragment of pP51 containing Tn5280 is cloned in the suicide donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida KT2442, TnS280 is found to transpose into the genome at random and in single copy. The presence of the catabolic genes tcbA and tcbB on TnS280 suggests a mechanism by which gene clusters can be mobilized as gene cassettes and joined with others to form novel catabolic pathways
Q52383; Q52384; Q52385; Q52386
the tecB gene is cloned into Escherichia coli DH5a together with the tecA gene into Escherichia coli DH5alpha
-