1.14.12.3: benzene 1,2-dioxygenase

This is an abbreviated version!
For detailed information about benzene 1,2-dioxygenase, go to the full flat file.

Reaction

Synonyms

BDO, BED, benzene dioxygenase, benzene hydroxylase, More, oxygenase, benzene 1,2-di-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

                1.14.12.3 benzene 1,2-dioxygenase

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Results	in table
171	AA Sequence
2	Application
1	CAS Registry Number
7	Cloned(Commentary)
18	Cofactor
2	KM Value [mM]
1	Localization
12	Metals/Ions
13	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
86	Organism
5	Pathway
3	pH Optimum
29	Protein Variants
3	Purification (Commentary)
2	Reaction
3	Reaction Type
21	Reference
9	Specific Activity [micromol/min/mg]
1	Storage Stability
51	Substrates and Products (Substrate)
11	Subunits
15	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.14.12.3 - benzene 1,2-dioxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli JM109

Pseudomonas putida

-

657604

expression in Escherichia coli JM109

Pseudomonas putida

-

439072

expression in Escherichia coli strain DH5alpha and JM109, co-expression with benzene dihydrodiol dehydrogenase under the control of the Ptac promoter or without any induction. The recombinant strains expressing the BED and the BDDH enzymes transform benzene into dihydrodiol with corresponding consumption of oxygen and regenerate NADH by converting dihydrodiol to catechol

Pseudomonas putida

-

711842

expression of the alpha-subunit and the beta-subunit of terminal dioxygenase in Escherichia coli

Pseudomonas putida

-

439071

expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and CJ236

Pseudomonas putida

-

657604

genes bedC1 and bedC2 encoding the terminal oxygenase alpha-subunit and beta-subunit, expression in Escherichia coli

Pseudomonas putida

-

439070

genes bedC1C2BA, under the control of the tac promoter, subcloned into pLAFR5, successfully conjugated into seven of the Gram-negative cis-1,2-cyclohexanediol-degrading isolates and stably maintained and expressed in three of them, strain CHD1–CHD3, the strains grow on cis-1,2-cyclohexanediol as sole carbon source, express an active BDO and oxidise cyclohexene, but none of the three strains is able to grow on cyclohexene as sole carbon source, overview, expression in Escherichia coli strain JM109

Pseudomonas putida

-

439072