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1.14.13.101: senecionine N-oxygenase

This is an abbreviated version!
For detailed information about senecionine N-oxygenase, go to the full flat file.

Reaction

senecionine
+
NADPH
 +
H+
 +
O2
=
senecionine N-oxide
 +
NADP+
 +
H2O

Synonyms

flavin-dependent monooxygenase, fmoa, GgPNO, oxygenase, senecionine N-, PA N-oxygenase, PNO, pyrrolizidine alkaloid N-oxygenase, senecionine monooxygenase (N-oxide-forming), senecionine N-oxygenase, SNO, TjSNO, ZvFMOa, ZvPNO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.101 senecionine N-oxygenase

Crystallization

Crystallization on EC 1.14.13.101 - senecionine N-oxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant isozyme PA N-oxygenase (ZvFMOa), mixing of 6.5 mg/ml protein in 20 mM glycine-NaOH, pH 9.0, and 1 mM DTT with an equal amount of precipitant solution containing 20 mM bis-tris propane-NaOH, pH 7.5, 200 mM NaNO3, 22% w/v PEG 3000, 1 mM TMA-HCl, and 1 mM NADP+, equilibration against reservoir solution, 18°C, X-ray diffraction structure determiantion and analysis at 3.0 A resolution
purified recombinant isozyme PA N-oxygenase (ZvPNO) with bound FAD or in complex with FAD and NADP+, hanging drop vapour diffusion method, mixing of 5.6 mg/ml protein in 20 mM glycine-NaOH, pH 9.0, and 1 mM DTT with an equal amount of precipitant solution containing 20 mM Tris-HCl, pH 7.0, 200 mM MgCl2, and 15% w/v PEG 3350, with or without 1 mM NADP+, equilibration against reservoir solution, 18°C, X-ray diffraction structure determiantion and analysis at 1.6-1.89 A resolution