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1.14.13.122: chlorophyllide-a oxygenase

This is an abbreviated version!
For detailed information about chlorophyllide-a oxygenase, go to the full flat file.

Word Map on EC 1.14.13.122

Reaction

71-hydroxychlorophyllide a
+
O2
 +
NADPH
 +
H+
=
chlorophyllide b
 + 2 H2O +
NADP+

Synonyms

AtPTC52, CAO, CAO1, Cao2, CAO_1, chlorophyll a oxygenase, chlorophyll a oxygenase 1, chlorophyll a oxygenase/chlorophyll b synthase, chlorophyllide a oxygenase, chlorophyllide a oxygenase 1, EC 1.13.12.14, fch2, OsCAO1, Pchlide a oxygenase, PGL, protochlorophyllide a oxygenase, ZmCAO1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.122 chlorophyllide-a oxygenase

Cloned

Cloned on EC 1.14.13.122 - chlorophyllide-a oxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis which overexpress a CAO–GFP fusion are mutagenized
-
construction of a genomic library, DNA and amino acid sequence determination
DNA and amino acid sequence determination and analysis of wild-type and mutants genes, gene structures, overview
-
expressed in Acaryochloris marina strain MBIC 11017
-
expressed in Arabidopsis thaliana and in Escherichia coli C41 cells
-
expressed in Nicotiana tabacum cultivar Petit Havana
-
expression analysis with isolated nuclei
expression in Arabidopsis thaliana
-
expression in Escherichia coli in membranes, phylogenetic tree
-
expression of GFP-tagged enzyme in Chlamydomonas reinhardtii cells, phylogenetic tree
-
expression of GFP-tagged wild-type and GFP-tagged domain mutant enzymes in wild-type and enzyme-deficient chlorin-1 mutant plants
-
expression of His-tagged enzyme in Escherichia coli
expression of His6-tagged CAO in Escherichia coli in inclusion bodies, in vitro expression by wheat germ lysates
-
expression of the enzyme under control of the 35S CaMV promoter, using the Agrobacterium tumefaciens infection system, in transgenic Arabidopsis thaliana plants renders the transgenic plants capable of normal rate photosynthetic growth also under low light conditions in contrast to the wild-type Arabidopsis thaliana plants, transgenic plants show altered chlorophyll a/b ratio, chloroplast structure, and thylakoid membrane composition
-
functional expression in Escherichia coli
functional overexpression in transgenic tobacco plants via Agrobacterium tumefaciens LBA4404 transfection, the CAO gene is expressed under control of the 35S CaMV promoter
-
gene CAO, DNA and amino acid sequence determination and analysis, genetic organization
gene pgl, recombinant expression of truncated protein OsCAO1pgl fused with GFP (35S::OsCAO1pgl-GFP) and transformed into Nicotiana benthamiana via Agrobacterium sp., specific expression in chloroplasts although the truncated enzyme lacks the signal sequence
gene ZmCAO1, bulked segregant analysis (BSA)-based mapping of the ZmCAO1 gene, quantitative real-time RT-PCR enzyme expression analysis
introduction of the Prochlorococcus gene into Synechocystis cells
-
introductionof the prokaryotic chlorophyll b synthesis gene for chlorophyllide a oxygenase (CAO) into Arabidopsis. In the transgenic plants (Prochlirothrix hollandica CAO plants), about 40% of chlorophyll a of the core antenna complexes is replaced by chlorophyll b in both photosystems
-
overexpression of the enzyme in Arabidopsis thaliana plants
-
pGreenII-0029 plasmid vector with fusion enzyme is introduced into Agrobacterium tumefaciens strain C58 by electroporation, wild-type and chlorina1-1 mutants of Arabidopsis thaliana are transformed by the infiltration with the bacterium, plant transformants are selected
-
plasmid vector pGreenII-0029 including the gene for the green fluorescent protein fused with full-length or truncated chlorophyllide a oxygenase, plasmid with fusion enzyme is then transformed into Agrobacterium tumefaciens (strain GV2260) by electroporation, wild-type Arabidopsis and chlorina1-1 mutant are transformed by vacuum infiltration
-
single-copy CAO gene, amino aid sequence and N-terminal extensions comparison with other higher plant sequences and the sequence of the algae Prochlorothrix hollandica, functional expression of ABC, BC, and C domain constructs in Synechocystis sp. PCC6803 reveals that the C domain is sufficient for catalytic activity
single-copy CAO gene, DNA and amino acid sequence determination and analysis, amino aid sequence and N-terminal extensions comparison with higher plant sequences, e.g. of Arabidopsis thaliana, functional expression in Synechocystis sp. PCC6803
-