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1.14.13.128: 7-methylxanthine demethylase

This is an abbreviated version!
For detailed information about 7-methylxanthine demethylase, go to the full flat file.

Word Map on EC 1.14.13.128

Reaction

7-methylxanthine
+
O2
+
NAD(P)H
+
H+
=
xanthine
+
NAD(P)+
+
H2O
+
formaldehyde

Synonyms

7-methylxanthine-specific N-demethylase, bacterial N-demethylase, caffeine demethylase, heteroxanthine demethylase, methylxanthine N-demethylase, N-demethylase, ndmA, NdmC, theobromine demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.128 7-methylxanthine demethylase

General Stability

General Stability on EC 1.14.13.128 - 7-methylxanthine demethylase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilization can be achieved by addition of either glycerol (20%), bovine serum albumin (10 mg/ml), dithiothreitol (1 mM) or the substrate 7-methylxanthine (1 mM). Storage under nitrogen atmosphere has a stabilizing influence. The addition of caffeine (1 mM), dimethylxanthines (1 mM) and protease inhibitors (pepstatin A, PMSF, 0.1 mM) is without influence on stability
-