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1.14.13.128: 7-methylxanthine demethylase

This is an abbreviated version!
For detailed information about 7-methylxanthine demethylase, go to the full flat file.

Word Map on EC 1.14.13.128

Reaction

7-methylxanthine
+
O2
 +
NAD(P)H
 +
H+
=
xanthine
 +
NAD(P)+
 +
H2O
 +
formaldehyde

Synonyms

7-methylxanthine-specific N-demethylase, bacterial N-demethylase, caffeine demethylase, heteroxanthine demethylase, methylxanthine N-demethylase, N-demethylase, ndmA, NdmC, theobromine demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.128 7-methylxanthine demethylase

Purification

Purification on EC 1.14.13.128 - 7-methylxanthine demethylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native heteroxanthine demethylase partially
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Sephacryl S200 gel filtration
purification of two-subunit N-demethylase component (Ndm) of the N-demethylase holoenzyme
-
recombinant His-tagged wild-type and mutant NdmC proteins by nickel affinity chromatography and gel filtration