220.127.116.11: 1-hydroxy-2-naphthoate hydroxylase
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1-hydroxy-2-naphthoic acid hydroxylase
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Purification on EC 18.104.22.168 - 1-hydroxy-2-naphthoate hydroxylase
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native enzyme 81fold by heat treatment at 60Â°C, ammonium sulfate fractionation and anion exchange chromatography. Additional purification steps, such as hydrophobic interaction chromatography or gel filtration, lead to the total or a significant, 70%, loss of activity, respectively, without achieving any further purification