Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.135: 1-hydroxy-2-naphthoate hydroxylase

This is an abbreviated version!
For detailed information about 1-hydroxy-2-naphthoate hydroxylase, go to the full flat file.

Reaction

Show molfile
1-hydroxy-2-naphthoate
+
Show molfile
NAD(P)H
+
Show molfile
H+
+
Show molfile
O2
=
Show molfile
1,2-dihydroxynaphthalene
+
Show molfile
NAD(P)+
+
Show molfile
H2O
+
Show molfile
CO2

Synonyms

1-hydroxy-2-naphthoic acid hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                EC 1.14.13.1351.14.13.135 1-hydroxy-2-naphthoate hydroxylase

Purification

Purification on EC 1.14.13.135 - 1-hydroxy-2-naphthoate hydroxylase

for references in articles please use BRENDA:EC1.14.13.135

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 81fold by heat treatment at 60┬░C, ammonium sulfate fractionation and anion exchange chromatography. Additional purification steps, such as hydrophobic interaction chromatography or gel filtration, lead to the total or a significant, 70%, loss of activity, respectively, without achieving any further purification
-