1.14.13.135: 1-hydroxy-2-naphthoate hydroxylase

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For detailed information about 1-hydroxy-2-naphthoate hydroxylase, go to the full flat file.

Reaction

Synonyms

1-hydroxy-2-naphthoic acid hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.135 1-hydroxy-2-naphthoate hydroxylase

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Results

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4

Cofactor

1

Expression

1

General Information

2

General Stability

4

KM Value [mM]

1

Metals/Ions

2

Molecular Weight [Da]

4

Natural Substrates/ Products (Substrates)

5

Organism

1

pH Optimum

1

Purification (Commentary)

1

Reaction

5

Reference

8

Source Tissue

3

Specific Activity [micromol/min/mg]

1

Storage Stability

6

Substrates and Products (Substrate)

1

Subunits

1

Synonyms

1

Systematic Name

1

Temperature Optimum [°C]

1

Temperature Stability [°C]

Purification

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Purification on EC 1.14.13.135 - 1-hydroxy-2-naphthoate hydroxylase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme 81fold by heat treatment at 60°C, ammonium sulfate fractionation and anion exchange chromatography. Additional purification steps, such as hydrophobic interaction chromatography or gel filtration, lead to the total or a significant, 70%, loss of activity, respectively, without achieving any further purification

Alcaligenes sp.

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712039