1.14.13.149: phenylacetyl-CoA 1,2-epoxidase
This is an abbreviated version!
For detailed information about phenylacetyl-CoA 1,2-epoxidase, go to the full flat file.
Word Map on EC 1.14.13.149
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1.14.13.149
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phenylacetic
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multicomponent
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burkholderia
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epoxide
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pa-coa
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phenylacetyl-coenzyme
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subcomplexes
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ligand-free
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cenocepacia
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di-iron
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cepir
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quorum
- 1.14.13.149
-
phenylacetic
-
multicomponent
- burkholderia
- epoxide
-
pa-coa
-
phenylacetyl-coenzyme
-
subcomplexes
-
ligand-free
- cenocepacia
-
di-iron
-
cepir
-
quorum
Reaction
Synonyms
PaaABCDE, PaaABCE, PaaAC, PaaAC(D), phenylacetyl-CoA monooxygenase, ring 1,2 phenylacetyl-CoA epoxidase
ECTree
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Crystallization
Crystallization on EC 1.14.13.149 - phenylacetyl-CoA 1,2-epoxidase
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hanging drop vapor diffusion method, in the absence of a bound ligand (with 100 mM N-(2-acetamido)-iminodiacetic acid pH 5.5) as well as in complexes with CoA, 3-hydroxybutyryl-CoA, benzoyl-CoA and phenylacetyl-CoA, using either 0.1 M PIPES pH 6.5, 15% (w/v) PEG 550 monomethyl ether or 0.1 M PIPES pH 6.5, 5% (v/v) 2-propanol, 5% (w/v) PEG 550 monomethyl ether
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hanging drop vapor diffusion method, PaaAC with acetyl-CoA is crystallized in 0.1 M sodium citrate buffer, pH 5.5, and 15% (w/v) PEG 6000 (Fluka). Crystals of ligand-free PaaAC are obtained in 100 mM N-(2-acetamido)-iminodiacetic acid, pH 5.5