amorpha-4,11-diene 12-monooxygenase

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amorpha-4,11-diene C-12 oxidase, amorpha-4,11-diene monooxygenase, CYP71AV1


     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
       amorpha-4,11-diene 12-monooxygenase


Cloned on EC - amorpha-4,11-diene 12-monooxygenase

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CLONED (Commentary)
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene CYP71AV1, quantitative real-time PCR enzyme expression analysis
gene cyp71av1, recombinant expression of cytochrome P450 CYP71AV1 in Saccharomyces cerevisiae, coexpression with artemisinic aldehyde D11(13) reductase (DBR2), and artemisinic aldehyde dehydrogenase (ALDH1), which recycles NADPH
gene CYP71AV1, semi-quantitative RT-PCR enzyme expression analysis, the expression level of CYP71AV1 significantly increases with the overexpression of AabHLH1