220.127.116.11: amorpha-4,11-diene 12-monooxygenase
This is an abbreviated version, for detailed information about amorpha-4,11-diene 12-monooxygenase, go to the full flat file.
transferred to EC 18.104.22.168
amorpha-4,11-diene C-12 oxidase, amorpha-4,11-diene monooxygenase, CYP71AV1
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Engineering on EC 22.214.171.124 - amorpha-4,11-diene 12-monooxygenase
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Saccharomyces cerevisiae expressing the native Artemisia annua cytochrome P450 monooxygenase (CYP71AV1) and artemisinic aldehyde D11(13) reductase (DBR2) is used as a whole-cell biocatalyst to produce the immediate artemisinin precursor, dihydroartemisinic acid (DHAA). Introducing artemisinic aldehyde dehydrogenase (ALDH1) from Artemisia annua, which recycles NADPH, results in a significant enhancement in artemisinate titer. Method optimization and evaluation, host screening and cofactor engineering, overview. The co-expression of DBR2 and modulation of abiotic conditions result in a higher yield. Optimal condition are 80 OD yeast cells in 0.05 ml reaction at pH 8.0. Growing the yeast cells at 20°C in galactose medium increases the dihydroartemisinate titer by 50%