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1.14.13.167: 4-nitrophenol 4-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.167

Reaction

4-nitrophenol
+
NADPH
 +
H+
 +
O2
=
1,4-benzoquinone
 +
nitrite
 +
NADP+
 +
H2O

Synonyms

2C4NP 4-monooxygenase, 4-NP/4-NCA monooxygenase, NpcA, para-nitrophenol 4-monooxygenase, PdcA, PNP 4-monooxygenase, pnpA, PnpA1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.167 4-nitrophenol 4-monooxygenase

Cloned

Cloned on EC 1.14.13.167 - 4-nitrophenol 4-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
-
gene pnpA, expression of the pnp cluster is detected only under inducing conditions, and pnpABA1CDEFG is a single transcriptional operon, genetic structure, RT-PCR and real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS
-
gene pnpA1, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS
-
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
the enzyme is encoded in the pnpABA1CDEF cluster, which is involved in para-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP) catabolism and is also likely responsible for 3-methyl-4-nitrophenol (3M4NP) degradation in strain SJ98, real-time quantitative PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS
-