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1.14.13.167: 4-nitrophenol 4-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.167

Reaction

4-nitrophenol
+
NADPH
 +
H+
 +
O2
=
1,4-benzoquinone
 +
nitrite
 +
NADP+
 +
H2O

Synonyms

2C4NP 4-monooxygenase, 4-NP/4-NCA monooxygenase, NpcA, para-nitrophenol 4-monooxygenase, PdcA, PNP 4-monooxygenase, pnpA, PnpA1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.167 4-nitrophenol 4-monooxygenase

Crystallization

Crystallization on EC 1.14.13.167 - 4-nitrophenol 4-monooxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol
structure in its apo and FAD-complex to a resolution of 2.04 A and 2.48 A, respectively. The 4-nitrophenol substrate lies near the N5 edge of isoalloxazine ring in the protein interior. The bound substrate is rotated 60° with respect to pOHB deviating from the isoalloxazine. The nitro group of 4-nitrophenol is directly hydrogen bonded to Val54 and Arg234, and the hydroxyl group is hydrogen bonded to Val223 and Cys23