188.8.131.52: dammarenediol 12-hydroxylase
This is an abbreviated version, for detailed information about dammarenediol 12-hydroxylase, go to the full flat file.
transferred to EC 184.108.40.206
CYP716A47, cytochrome P450-type protopanaxadiol synthase, PPDS, PqD12H, protopanaxadiol synthase
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Cloned on EC 220.127.116.11 - dammarenediol 12-hydroxylase
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gene CYP716A47, recombinant expression under control of 35S CaMV promoter in leaves of tobacco via Agrobacterium tumefaciens GV3101 transformation method, RT-PCR expression analysis
gene PqD12H, expression of PqD12H in Saccharmoyces cerevisiae strain WAT21 from plasmid pAUR123-PqD12H, RT-PCR enzyme expression analysis, recombinant coexpression with dammarenediol synthase DS leads to increased accumulation of ginsenosides, i.e. protopanaxatriol protopanaxadiol. compared to control, both PqDS and PqD12H have higher accumulation of mRNA in the transgenic hairy roots and the increase ratio decreased slightly over culture time. Yeast with single recombinant vector pAUR123-PqD12H is unable to express protopanaxadiol without exogenous dammarenediol-II
recombinant expression of wild-type and mutant fusion proteins in Saccharomyces cerevisiae strain W303-1a. coexpression of wild-type enzyme with dammarenediol-II synthase from Panax ginseng and a Arabidopsis thaliana NADPH-cytochrome P450 reductase (ATR1). The low metabolic flux through PPDS results in a low productivity of protopanaxadiol, but is increased with the fusion enzymes