188.8.131.52: dammarenediol 12-hydroxylase
This is an abbreviated version, for detailed information about dammarenediol 12-hydroxylase, go to the full flat file.
transferred to EC 184.108.40.206
CYP716A47, cytochrome P450-type protopanaxadiol synthase, PPDS, PqD12H, protopanaxadiol synthase
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Engineering on EC 220.127.116.11 - dammarenediol 12-hydroxylase
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to increase the low metabolic flux through PPDS and increase the productivity of protopanaxadiol production, enzyme PPDS is modified through transmembrane domain truncation and construction of self-sufficient PPDS-ATR1 fusion proteins. The fusion enzymes exhibit approximately 4.5fold increase in catalytic activity and 71.1% increase in protopanaxadiol production compared with PPDS and ATR1 co-expression. The engineered yeast carrying fusion protein effectively converts 96.8% of dammarenediol-II into protopanaxadiol, bioreactor in fed-batch fermentation. Construction of four fusion proteins named as PPDS-linker1-46tATR1, PPDSlinker2-46tATR1, PPDS-linker3-46tATR1, and PPDS-nolinker-46tATR1, respectively. In addition, 31tPPDS heme domain and 46tATR1 reductase domain are linked by polypeptide GSTSSGSG. Method optimization, overview. Evaluation of the oxidative stress in yeast cells induced by co-expression of PPDS and ATR1