126.96.36.199: L-evernosamine nitrososynthase
This is an abbreviated version!
For detailed information about L-evernosamine nitrososynthase, go to the full flat file.
Word Map on EC 188.8.131.52
+ 2 NADPH + 2 H+ + 2 O2 = + 2 NADP+ + 3 H2O
DnmZ, nitrososynthase, ORF36
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Advanced search results
Crystallization on EC 184.108.40.206 - L-evernosamine nitrososynthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
to 3.15 A resolution. Tetrameric enzyme that shares a fold with acyl-CoA dehydrogenases and class D flavin-containing monooxygenases, including the nitrososynthase KijD3Enzym displays an unusually open active site. Modeling of FAD and TDP-L-evernosamine into the active site
purified enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml protein solution containing 6 mg/ml of protein DnmZ with 0.001 ml crystallization solution containing 0.05 M glycine pH 9.5, 0.2 M ammonium sulfate, 10% w/v, and PEG 4000, and equilibration against 0.75 ml crystallization solution, 26Â°C, X-ray diffraction structure determination and analysis at 2.74-3.0 A resolution, molecular replacement using crystal structure PDB ID 3mxl as search model. Cocrystallization of DnmZ complexed with dTDP-L-epi-vancosamine, FAD, FMN or combinations of the amino sugar and flavin are unsuccessful