1.14.13.187: L-evernosamine nitrososynthase

This is an abbreviated version!
For detailed information about L-evernosamine nitrososynthase, go to the full flat file.

Reaction

Synonyms

DnmZ, nitrososynthase, ORF36

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.187 L-evernosamine nitrososynthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

All data fields related to EC 1.14.13.187

Results	in table
3	AA Sequence
7	Cofactor
2	Crystallization (Commentary)
3	General Information
3	Natural Substrates/ Products (Substrates)
8	Organism
8	PDB ID
10	Reaction
8	Reference
13	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
1	Systematic Name

Crystallization

Crystallization on EC 1.14.13.187 - L-evernosamine nitrososynthase

for references in articles please use BRENDA:EC1.14.13.187

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

to 3.15 A resolution. Tetrameric enzyme that shares a fold with acyl-CoA dehydrogenases and class D flavin-containing monooxygenases, including the nitrososynthase KijD3Enzym displays an unusually open active site. Modeling of FAD and TDP-L-evernosamine into the active site

Micromonospora carbonacea

B5APQ9

726951

purified enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml protein solution containing 6 mg/ml of protein DnmZ with 0.001 ml crystallization solution containing 0.05 M glycine pH 9.5, 0.2 M ammonium sulfate, 10% w/v, and PEG 4000, and equilibration against 0.75 ml crystallization solution, 26°C, X-ray diffraction structure determination and analysis at 2.74-3.0 A resolution, molecular replacement using crystal structure PDB ID 3mxl as search model. Cocrystallization of DnmZ complexed with dTDP-L-epi-vancosamine, FAD, FMN or combinations of the amino sugar and flavin are unsuccessful

Streptomyces peucetius

A0A0R4I990

743958