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1.14.13.19: taxifolin 8-monooxygenase

This is an abbreviated version!
For detailed information about taxifolin 8-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.19

Reaction

taxifolin
+
NAD(P)H
+
H+
+
O2
=
2,3-dihydrogossypetin
+
NAD(P)+
+
H2O

Synonyms

F8H, flavin monooxygenase-like flavonoid 8-hydroxylase, flavonoid 8-hydroxylase, LjF8H, taxifolin hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.19 taxifolin 8-monooxygenase

Cloned

Cloned on EC 1.14.13.19 - taxifolin 8-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene LjB13E21 or LjF8H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant enzyme expression in Arabidopsis thaliana strain Col-0 seedlings under the control of a CaMV35S promoter using a pBE2113 vector via transfection method using Agrobacterium tumefaciens strain (GV3101::pMP90), and recombinant expression in Saccharomyces cerevisiae strain BJ2168 microsomes, semi-quantitative RT-PCR enzyme expression analysis