1.14.13.195: L-ornithine N5-monooxygenase (NADPH)

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For detailed information about L-ornithine N5-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.195

1.14.13.195

flavin

fumigatus

aspergillus

flavin-dependent

pyoverdine

nadp+

c4a-hydroperoxyflavin

hydroxamate

hydroxamate-containing

flavin-containing

non-ribosomal

half-reaction

n-hydroxylating

Reaction

Synonyms

Af-OMO, CchB, flavin-dependent monooxygenase, L-Orn N5-oxygenase, L-ornithine N5-hydroxylase, L-ornithine-Ndelta-oxygenase, monooxygenase EtcB, omega-amino acid monooxygenase, ornithine hydroxylase, ornithine N5-monooxygenase, PsbA, PvdA, SidA, siderophore A

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.195 L-ornithine N5-monooxygenase (NADPH)

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Results	in table
3	Activating Compound
86	AA Sequence
1	CAS Registry Number
13	Cloned(Commentary)
33	Cofactor
2	Crystallization (Commentary)
7	Expression
10	General Information
7	Inhibitors
19	kcat/KM [mM/s]
4	Ki Value [mM]
27	KM Value [mM]
3	Localization
1	Metals/Ions
2	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
69	Organism
4	Pathway
8	pH Optimum
3	pH Range
1	pI Value
14	Posttranslational Modification
9	Protein Variants
5	Purification (Commentary)
3	Reaction
23	Reference
3	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
4	Subunits
37	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
15	Turnover Number [1/s]

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Cloned on EC 1.14.13.195 - L-ornithine N5-monooxygenase (NADPH)

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Aspergillus fumigatus

Q5SE95

725540

expression as His6-tagged protein in Escherichia coli

Saccharopolyspora erythraea

714263

expression of PvdA-PhoA fusion protein mutants in Escherichia coli strain LMG194

Pseudomonas aeruginosa

705555

gene cchB, overexpression of N-terminally thioredoxin-tagged enzyme in Escherichia coli strain BL21(DE3)

Streptomyces coelicolor

706031

gene psbA, DNA and amino acid sequence determination and analysis, monocistronic gene

Pseudomonas sp.

Q9F8X0

704262

gene pvdA, expression of His-tagged enzyme in Escherichia coli strain Rosetta2(DE3)

Pseudomonas aeruginosa

704269

gene sidA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)-T1

Aspergillus fumigatus

E9QYP0

744163

PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons

5 entries

PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons, promoter activity analysis during cell growth. Recombinant expression of N-terminally His-tagged full-length and truncated wild-type and mutant enzymes in Escherichia coli strain M15

Pseudomonas aeruginosa

Q51548

702009