1.14.13.195: L-ornithine N5-monooxygenase (NADPH)

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For detailed information about L-ornithine N5-monooxygenase (NADPH), go to the full flat file.

Reaction

Synonyms

Af-OMO, CchB, flavin-dependent monooxygenase, L-Orn N5-oxygenase, L-ornithine N5-hydroxylase, L-ornithine-Ndelta-oxygenase, monooxygenase EtcB, omega-amino acid monooxygenase, ornithine hydroxylase, ornithine N5-monooxygenase, PsbA, PvdA, SidA, siderophore A

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.195 L-ornithine N5-monooxygenase (NADPH)

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Results	in table
3	Activating Compound
86	AA Sequence
1	CAS Registry Number
13	Cloned(Commentary)
33	Cofactor
2	Crystallization (Commentary)
7	Expression
10	General Information
7	Inhibitors
19	kcat/KM [mM/s]
4	Ki Value [mM]
27	KM Value [mM]
3	Localization
1	Metals/Ions
2	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
69	Organism
4	Pathway
8	pH Optimum
3	pH Range
1	pI Value
14	Posttranslational Modification
9	Protein Variants
5	Purification (Commentary)
3	Reaction
23	Reference
3	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
4	Subunits
37	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
15	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.195 - L-ornithine N5-monooxygenase (NADPH)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Saccharopolyspora erythraea

-

714263

recombinant His-tagged enzyme from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin

Pseudomonas aeruginosa

-

704269

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-T1 by affinity chromatography, proteolytic tag removal

Aspergillus fumigatus

E9QYP0

744163

recombinant N-terminally His-tagged full-length and truncated wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography

Pseudomonas aeruginosa

Q51548

702009

recombinant N-terminally thioredoxin-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Streptomyces coelicolor

-

706031