1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.2

Reaction

4-hydroxybenzoate
+
NADPH
+
H+
+
O2
=
3,4-dihydroxybenzoate
+
NADP+
+
H2O

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, BxeA2040, HBH, m-hydroxybenzoate hydroxylase, MobA, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PobA, pobACg, POHBase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.2 4-hydroxybenzoate 3-monooxygenase

Inhibitors

Inhibitors on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-O-gallate
-
non-competitive, binds to the enzyme in the proximity of the FAD binding site via formation of three hydrogen bonds
2,4-Dihydroxybenzoate
-
competitive with 4-hydroxybenzoate
3,4-dihydroxybenzoate
3-Chloro-4-hydroxybenzoate
-
mixed-type
3-hydroxybenzoate
4-Aminobenzoate
4-Aminosalicylate
-
competitive
4-Fluorobenzoate
4-hydroxy-3-nitrobenzoic acid
-
2 mM, 23% inhibition
4-hydroxybenzaldehyde
4-hydroxybenzoate
4-hydroxybutyrate
-
92 mM, pH 8, very slight inhibition
4-hydroxycinnamate
-
-
4-hydroxyphenylacetic acid
-
2 mM, 16% inhibition
4-nitrophenol
-
2 mM, 39% inhibition
6-Aminonicotinate
-
competitive
6-Hydroxynicotinate
acetate
-
92 mM, pH 8, very slight inhibition
Benzoate
citrate
-
92 mM, pH 8, very slight inhibition
coumaric acid
-
2 mM, 30% inhibition
Cu2+
-
1 mM, moderate inhibition
diethyl dicarbonate
Fe3+
-
1 mM, moderate inhibition
formate
-
92 mM, pH 8, very slight inhibition
fumarate
-
92 mM, pH 8, very slight inhibition
Hg2+
-
0.1 mM HgCl2, complete inhibition
iodoacetamide
-
reversed by dithiothreitol
Maleate
-
92 mM, pH 8, very slight inhibition
Mn2+
-
1 mM, moderate inhibition
n-dodecyl gallate
-
non-competitive
N-iodosuccinimide
-
reversed by dithiothreitol
NEM
-
0.1 mM, 59% inhibition
o-Iodosobenzoate
-
reversed by dithiothreitol
p-hydroxy-3-iodomethylbenzoate
-
1 mM, irreversible crosslinking to the substrate binding site
Phenylglyoxal
-
pseudo-first order kinetics, incorporation into the substrate-binding site
phosphate
propionate
-
92 mM, pH 8, very slight inhibition
protocatechuate
-
above 1 mM
salicylate
Tartrate
-
92 mM, pH 8, very slight inhibition
additional information
-