1.14.13.210: 4-methyl-5-nitrocatechol 5-monooxygenase

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For detailed information about 4-methyl-5-nitrocatechol 5-monooxygenase, go to the full flat file.

4-methyl-5-nitrocatechol monooxygenase, 4-methyl-5-nitrocatechol oxygenase, 4M5NC monooxygenase, dntB, MNC, MNC monooxygenase, MNC oxygenase

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Results	in table
6	AA Sequence
2	Cloned(Commentary)
6	Cofactor
4	General Information
1	Inhibitors
2	kcat/KM [mM/s]
3	KM Value [mM]
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
8	Organism
3	Pathway
1	pH Optimum
10	Protein Variants
2	Purification (Commentary)
3	Reaction
6	Reference
1	Specific Activity [micromol/min/mg]
18	Substrates and Products (Substrate)
2	Subunits
19	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Purification on EC 1.14.13.210 - 4-methyl-5-nitrocatechol 5-monooxygenase

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native enzme to homogeneity from strain DNT by anion exchange chromatography and gel filtration

P71029

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis