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1.14.13.217: protodeoxyviolaceinate monooxygenase

This is an abbreviated version!
For detailed information about protodeoxyviolaceinate monooxygenase, go to the full flat file.

Reaction

protodeoxyviolaceinate
+
NAD(P)H
+
O2
=
protoviolaceinate
+
NAD(P)+
+
H2O

Synonyms

protoviolaceinate synthase, VioD, VioD oxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.217 protodeoxyviolaceinate monooxygenase

Purification

Purification on EC 1.14.13.217 - protodeoxyviolaceinate monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) to homogeneity by nickel affinity chromatography and gel filtration in tandem