1.14.13.22: cyclohexanone monooxygenase
This is an abbreviated version!
For detailed information about cyclohexanone monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.22
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1.14.13.22
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baeyer-villiger
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acinetobacter
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lactones
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bvmos
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ncimb
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calcoaceticus
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cyclohexanol
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synthesis
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criegee
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epsilon-caprolactone
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c4a-peroxyflavin
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biooxidation
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bicyclo3.2.0hept-2-en-6-one
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phenylacetone
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cyclopentanone
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biotechnology
- 1.14.13.22
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baeyer-villiger
- acinetobacter
- lactones
-
bvmos
-
ncimb
- calcoaceticus
- cyclohexanol
- synthesis
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criegee
- epsilon-caprolactone
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c4a-peroxyflavin
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biooxidation
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bicyclo3.2.0hept-2-en-6-one
- phenylacetone
- cyclopentanone
- biotechnology
Reaction
Synonyms
Bpro_556, CAMO, CHMO, ChnB, chnB protein, chnB1 protein, CHXON, CMO, cycloalkaone monooxygenase, cyclohexanone 1, 2-mono-oxygenase, cyclohexanone mono-oxygenase, cyclohexanone monooxygenase, cyclohexanone oxygenase, cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming), oxygenase, cyclohexanone mono-
ECTree
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Specific Activity
Specific Activity on EC 1.14.13.22 - cyclohexanone monooxygenase
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0.4
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substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, modified Riesenberg medium, 30°C
0.5
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substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, BHI medium, 30°C
0.85
mutant W492A, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C
2.1
6
wild type enzyme, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C
9.1
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purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one
9.2
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purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one
additional information
additional information
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activity is dependent on substrate concentration and buffer conditions
additional information
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activity below 0.1 micromol/min/mg protein, substrate cyclohexanone, induced (0.1 mM isopropylthiogalactoside) cell culture extracts, BHI medium or modified Riesenberg medium, 30°C
additional information
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whole cell activity to produce epsilon-caprolactone from cyclohexanone: initially 72 micromol/min/g dry cells, linearly falling to 14-18 micromol/min/g dry cells, sodium-potassium phosphate buffer, pH 7.0 with glucose (transferred from modified Riesenberg medium used for cultivation), 30°C