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1.14.13.22: cyclohexanone monooxygenase

This is an abbreviated version!
For detailed information about cyclohexanone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.22

Reaction

cyclohexanone
+
NADPH
+
H+
+
O2
=
hexano-6-lactone
+
NADP+
+
H2O

Synonyms

Bpro_556, CAMO, CHMO, ChnB, chnB protein, chnB1 protein, CHXON, CMO, cycloalkaone monooxygenase, cyclohexanone 1, 2-mono-oxygenase, cyclohexanone mono-oxygenase, cyclohexanone monooxygenase, cyclohexanone oxygenase, cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming), oxygenase, cyclohexanone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.22 cyclohexanone monooxygenase

Temperature Stability

Temperature Stability on EC 1.14.13.22 - cyclohexanone monooxygenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
mutant T415C is the most stable variant with a 30fold increased long-term stability, 33% residual activity after 24 h incubation at 25°C
31.6
Tm-value of wilde-type enzyme
36
melting temperature of wild-type
36.4
Tm-value of mutant enzyme T415C/A463C
37.5
Tm-value of mutant enzyme T415C
38
Tm-value of wild-type enzyme
40
-
2 min, 40% loss of activity for isozyme type 1, 7% loss of activity for isozyme type 2
40.5
Tm-value of mutant enzymes A255C/A293C and A325C/L483C
44
Tm-value of mutant enzyme L323C/A325C
48.8
melting temperature of mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R