1.14.13.23: 3-hydroxybenzoate 4-monooxygenase
This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.23
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1.14.13.23
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phenol
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4-hydroxybenzoate
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hydroxylases
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testosteroni
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fad
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gentisate
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comamonas
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mobility-shift
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salicylate
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repressor
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regioselective
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flavoprotein
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unbound
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mobr
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dioxygenase
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protocatechuate
- 1.14.13.23
- phenol
- 4-hydroxybenzoate
- hydroxylases
- testosteroni
- fad
- gentisate
-
comamonas
-
mobility-shift
- salicylate
- repressor
-
regioselective
- flavoprotein
-
unbound
- mobr
- dioxygenase
- protocatechuate
Reaction
Synonyms
3-hydroxybenzoate 4-hydroxylase, 3-hydroxybenzoate hydroxylase, 3-hydroxybenzoate-4-hydroxylase, 3HB4H, EC 1.14.99.13, FAD-dependent 4-hydroxybenzoate hydroxylase, m-hydroxybenzoate hydroxylase, MHBH, MobA, oxygenase, 3-hydroxybenzoate 4-mono-
ECTree
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Engineering
Engineering on EC 1.14.13.23 - 3-hydroxybenzoate 4-monooxygenase
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A400G
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol
D416A
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol
H135P
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol
K326I
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol
V257A
additional information
screening of random mutants from a cosmid library for altered substrate specificities
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol, the mutant is also active with resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol
V257A
a directed evolution study reveals that 3HB4H from Comamonas testosteroni strain GZ39 is considerably active with 4-hydroxybenzoate and that this activity increases in the V257A variant. The same variant slowly converts phenol to catechol, an activity not observed with the wild-type enzyme