1.14.13.24: 3-hydroxybenzoate 6-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.24

1.14.13.24

gentisate

flavoprotein

flavin

fad

rhodococcus

salicylate

hydroxylases

jostii

2,5-dihydroxybenzoate

maleylpyruvate

para-hydroxylation

alcaligenes

nadh-dependent

flavin-dependent

ncimb

near-uv

fumarylpyruvate

nadh-specific

p-hydroxybenzoate

regioselective

Reaction

Synonyms

3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.24 3-hydroxybenzoate 6-monooxygenase

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Results	in table
4	Activating Compound
1676	AA Sequence
1	Application
1	CAS Registry Number
10	Cloned(Commentary)
48	Cofactor
1	Crystallization (Commentary)
15	General Information
2	General Stability
26	Inhibitors
14	kcat/KM [mM/s]
44	KM Value [mM]
2	Localization
3	Metals/Ions
19	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
47	Organism
7	Pathway
5	PDB ID
13	pH Optimum
6	pH Range
1	pH Stability
2	Posttranslational Modification
19	Protein Variants
14	Purification (Commentary)
7	Reaction
4	Reaction Type
33	Reference
4	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
80	Substrates and Products (Substrate)
19	Subunits
41	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
3	Temperature Range [°C]
1	Temperature Stability [°C]
19	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase

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2 entries

98% homogeneity

Micrococcus sp.

438961

DEAE-Sepharose column chromatography and Sephadex G-25 gel filtration

Rhodococcus jostii

726983

homogeneity

3 entries

Ni-NTA agarose column chromatography and Q-Sepharose column chromatography

Rhodococcus jostii

727077

recombinant His-tagged NarX from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography to homogeneity

Rhodococcus sp.

E7CYP8

710986

recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Martelella sp. AD-3

764754

recombinant N-terminally His-tagged Ncgl2923 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity

Corynebacterium glutamicum

Q8NLB6

712313

recombinnat His-tagged enzyme from Escherichia coli by metal chelating chromatography

Pseudomonas alcaligenes

Q9F131

674255

single step purification using substrate-mediated interaction of the enzyme with blue-Sepharose, homogeneity

Micrococcus sp.

438957