1.14.13.24: 3-hydroxybenzoate 6-monooxygenase
This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.24
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1.14.13.24
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gentisate
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flavoprotein
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flavin
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fad
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rhodococcus
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salicylate
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hydroxylases
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jostii
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2,5-dihydroxybenzoate
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maleylpyruvate
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para-hydroxylation
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alcaligenes
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nadh-dependent
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flavin-dependent
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ncimb
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near-uv
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fumarylpyruvate
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nadh-specific
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p-hydroxybenzoate
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regioselective
- 1.14.13.24
- gentisate
- flavoprotein
- flavin
- fad
- rhodococcus
- salicylate
- hydroxylases
- jostii
- 2,5-dihydroxybenzoate
- maleylpyruvate
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para-hydroxylation
- alcaligenes
-
nadh-dependent
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flavin-dependent
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ncimb
-
near-uv
- fumarylpyruvate
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nadh-specific
- p-hydroxybenzoate
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regioselective
Reaction
Synonyms
3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD
ECTree
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Storage Stability
Storage Stability on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase
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-80°C, purified recombinant enzyme, 50 mM MOPS or 100 mM potassium phosphate, 10% v/v glycerol, 0.2 mM FAD, 60% activity remaining after 3 months
22°C, rom temperature, purified recombinant enzyme, 50 mM MOPS or 100 mM potassium phosphate, 10% v/v glycerol, 0.2 mM FAD, complete loss of activity after 12 h
4°C, optimal storage in 50 mM phosphate or Tris buffer pH 8 with 10% glycerol
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4°C, purified recombinant enzyme, 50 mM MOPS or 100 mM potassium phosphate, 10% v/v glycerol, 0.2 mM FAD, complete loss of activity after 3 days