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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
+
H+
+
O2
=
methanol
+
NAD(P)+
+
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

Engineering

Engineering on EC 1.14.13.25 - methane monooxygenase (soluble)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G10A/G13Q/G16A
G10A/G13Q/G16A
A115C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
A62C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview, the mutant MMOH-MMOB complex is perturbed by salts but not nonionic detergents
D71C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
D87C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
G119C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
H33A
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis, an N-terminal region variant, structure analysis
H5A
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis, an N-terminal region variant, structure analysis
K44C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
L110C
-
mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme
L110G
-
mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme
L110R
-
mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme
L110Y
-
mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl and ethylbenzene as a substrate compared to the wild type enzyme
N107G/S109A/S110A/T111A
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis, mutations in the core region, termed the Quad variant, structure analysis
N107G/S110A
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis, a binary derivative of the Quad variant, termed DBL1. The DBL1 mutation in MMOB leads to a loss of the S110 hydrogen bond with N214 of the sMMOH alpha-subunit, structure analysis
R133C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
S109A/T111A
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis, a binary derivative of the Quad variant, termed DBL2, structure analysis
S109C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
T111C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
T111Y
V39C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
V39F
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component, the mutant component variant nearly halts the reaction of the reconstituted sMMO system
V39R
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component, the mutant component variant nearly halts the reaction of the reconstituted sMMO system
V41E
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component, the mutant component variant nearly halts the reaction of the reconstituted sMMO system
V41F
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component, the mutant component variant nearly halts the reaction of the reconstituted sMMO system
V41R
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component, the mutant component variant nearly halts the reaction of the reconstituted sMMO system
V68C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
W77F
A0A2D2D5X0; A0A2D2D0T8; Q53563; A0A2D2D0X7
site-directed mutagenesis in the MMOB component
Y102C
-
site-directed mutagenesis of enzyme component MMOH, mobility and accessibility parameters for the spin-labeled MMOB mutants alone and in complex with MMOH in comparison to the wild-type enzyme, overview
additional information