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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
+
H+
+
O2
=
methanol
+
NAD(P)+
+
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

Metals Ions

Metals Ions on EC 1.14.13.25 - methane monooxygenase (soluble)

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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
-
the enzyme expresses the soluble enzyme form under copper limitation, and the membrane-bound particulate MMO at high copper-to-biomass ratio, mechanism of the copper switch involves a tetrameric 480 kDA sensor protein MmoS, encoded by gene mmoS, as part of a two-component signaling system, domain organization, MmoS contains a FAD cofactor, indirect regulation without binding of copper to MmoS, overview
Fe3+
P22869; P18798; P11987; P18797; P22868; P22867
the enzyme has diiron (FeIII-FeIII) active sites where different types of hydrocarbons are oxidized through orchestrated hydroxylase, regulatory and reductase components for precise control of hydrocarbons, oxygen, protons, and electrons
NaCl
-
as a true halotolerant enzyme, MmoC still shows 50% of its specific activity at 2 M NaCl. Evaluation of salt tolerance for NADH-mediated reduction of benzyl viologen by MmoC at non-optima conditions, 23°C and pH 7.0
Ni2+
-
protein B contains 0.04 mol Ni2+ per mol protein
[2Fe-2S] cluster
-
bound to the MMOR enzyme component
additional information