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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
 +
H+
 +
O2
=
methanol
 +
NAD(P)+
 +
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

Storage Stability

Storage Stability on EC 1.14.13.25 - methane monooxygenase (soluble)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, component A, stable for several weeks
-
-80°C, more than 1 year
-
0°C, protein C, loss of 60-90% of activity within 20 h
-
0°C, proteins A and B, stable for at least 24 h
-
4°C, sMMO component C reductase, 90% loss of activity after 120 h, can be restored by Fe2+
-
sMMO component A hydroxylase is very unstable, splits into inactive subunits when stored frozen even for short periods
-
sMMO component protein B is stable when stored frozen
-