1.14.13.29: 4-nitrophenol 2-monooxygenase

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For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.29 4-nitrophenol 2-monooxygenase

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Results	in table
4	Activating Compound
57	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
17	Cofactor
2	Crystallization (Commentary)
131	Disease/ Diagnostics
28	General Information
1	General Stability
4	IC50 Value
27	Inhibitors
2	Ki Value [mM]
4	KM Value [mM]
11	Localization
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
26	Organism
3	Pathway
7	pH Optimum
21	Protein Variants
5	Purification (Commentary)
4	Reaction
3	Reaction Type
23	Reference
16	Source Tissue
15	Specific Activity [micromol/min/mg]
2	Storage Stability
45	Substrates and Products (Substrate)
10	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Homo sapiens

-

438908

DNA and amino acid sequence determination and analysis, phylogenetic analysis

Serratia sp. DS001

-

684593

PCR-amplification, shuttle vector pK4 is used to construct pKPN plasmids containing enzyme gene and reporter gene, hosts are Rhodococcus sp. PN1 and Rhodococcus rhodochrous ATCC 12674 (electroporation), Escherichia coli JM109 is host for propagation and purification of recombinant plasmids

Rhodococcus sp. PN1

Q8RQQ0

698597

recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)/pLysS

Rhodococcus opacus

-

763974