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1.14.13.39: nitric-oxide synthase (NADPH)

This is an abbreviated version!
For detailed information about nitric-oxide synthase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.39

Reaction

2 L-arginine + 2 NADPH + 2 H+ + 2 O2 = 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O

Synonyms

bacterial nitric oxide synthase, bacterial nitric-oxide synthase, bNOS, bsNOS, cb-NOS, cytokine inducible NOS, DNOS, e-NOS, EC-NOS, endothelial nitric oxide synthase, endothelial nitric-oxide synthase, endothelial NO synthase, endothelial NOS, endothelium-derived relaxation factor-forming enzyme, endothelium-derived relaxing factor synthase, eNOS, i-NOS, inducible nitric oxide synthase, inducible nitric-oxide synthase, inducible NO synthase, inducible NOS, iNOS, mitochondrial NO synthase, mitochondrial-specific nitric oxide synthase, mtNOS, n-NOS, NADPH diaphorase, NADPH-d, NADPH-diaphorase, NADPHd, neuronal nitric oxide synthase, neuronal nitric-oxide synthase, neuronal NO synthase, neuronal NOS, nicotinamide adenine dinucleotide phosphate-diaphorase, nitric oxid synthase, nitric oxide synthase, nitric oxide synthase-like protein, nitric oxide synthetase, nitric-oxide synthase, nNOS, nNOSalpha, NO synthase, NO synthase type I, NO synthase type II, NO synthase type III, NO-synthase, NOS, NOS I, NOS-2, NOS1, NOS2, NOS3, synthetase, nitric oxide

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.39 nitric-oxide synthase (NADPH)

Specific Activity

Specific Activity on EC 1.14.13.39 - nitric-oxide synthase (NADPH)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000000335
-
crude extract
0.000000305
-
crude extract
0.000009
-
liver mitochondria
0.000017
-
liver
0.000033
-
brain mitochondria
0.000043
-
crude extract
0.000073
-
purified enzyme, without tetrahydropterin
0.00035
-
purified enzyme, with tetrahydropterin
0.00054
-
brain
0.00074
-
crude extract
0.0075
-
brain homogenates
0.0098
-
purified enzyme
0.01
-
reductase activity
0.031
0.0353
-
pH 6.5, 47.5°C
0.074
-
purified enzyme
0.142
-
wild-type
0.143
-
purified recombinant enzyme
0.159
-
mutant E298D
0.181
-
partially purified enzyme
0.19
-
purified recombinant enzyme
0.34 - 0.35
0.41
-
substrate Ngamma-hydroxy-L-arginine, purified enzyme
0.6
-
pH 7.4, 30°C
0.73
-
purified enzyme
0.815
-
purified recombinant enzyme, H2O2-supported reduction of Ngamma-hydroxy-L-arginine, with tetrahydrobiopterin, 25°C
0.94 - 0.96
1
-
purified enzyme
1.06
-
purified enzyme
1.1
-
purified enzyme
1.62
-
purified enzyme
1.9
-
purified enzyme
38
-
purified enzyme, inducible isoform, cytochrome c reductase activity
983.7
-
pH 7.0, 37°C
additional information