1.14.13.44: 2-hydroxybiphenyl 3-monooxygenase

This is an abbreviated version!
For detailed information about 2-hydroxybiphenyl 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.44

1.14.13.44

azelaica

phenol

fad

2-substituted

flavin

2,3-dihydroxybiphenyl

biocatalytic

non-substrate

laboratory-evolved

amberlite

fad-dependent

entrance

ortho-hydroxylation

flavoprotein

catechols

synthesis

Reaction

Synonyms

2-hydroxybiphenyl 3-monooxygenase, HBP1, HbpA, oxygenase, 2-hydroxybiphenyl 3-mono-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.44 2-hydroxybiphenyl 3-monooxygenase

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Results	in table
3	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
17	Cofactor
5	Crystallization (Commentary)
4	General Information
10	Inhibitors
24	kcat/KM [mM/s]
1	Ki Value [mM]
41	KM Value [mM]
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
35	Organism
2	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
25	Protein Variants
6	Purification (Commentary)
3	Reaction
5	Reaction Type
16	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
54	Substrates and Products (Substrate)
4	Subunits
12	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
44	Turnover Number [1/s]

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Reference on EC 1.14.13.44 - 2-hydroxybiphenyl 3-monooxygenase

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438847

Meyer, A.; Schmid, A.; Held, M.; Westphal, A.H.; Rothlisberger, M.; Kohler, H.P.E.; Van Berkel, W.J.H.; Witholt, B.

Changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from Pseudomonas azelaica HBP1 by directed evolution

J. Biol. Chem.

277

5575-5582

2002

Pseudomonas nitroreducens, Pseudomonas nitroreducens HBP1

11733527

438848

Meyer, A.; Wursten, M.; Schmid, A.; Kohler, H.P.; Witholt, B.

Hydroxylation of indole by laboratory-evolved 2-hydroxybiphenyl 3-monooxygenase

J. Biol. Chem.

277

34161-34167

2002

Pseudomonas nitroreducens, Pseudomonas nitroreducens HBP1

12105208

438849

Suske, W.A.; Van Berkel, W.J.H.; Kohler, H.P.E.

Catalytic mechanism of 2-hydroxybiphenyl 3-monooxygenase, a flavoprotein from Pseudomonas azelaica HBP1

J. Biol. Chem.

274

33355-33365

1999

Pseudomonas nitroreducens, Pseudomonas nitroreducens HBP1

10559214

438850

Suske, W.A.; Held, M.; Schmid, A.; Fleischmann, T.; Wubbolts, M.G.; Kohler, H.P.E.

Purification and characterization of 2-hydroxybiphenyl 3-monooxygenase, a novel NADH-dependent, FAD-containing aromatic hydroxylase from Pseudomonas azelaica HBP1

J. Biol. Chem.

272

24257-24265

1997

Pseudomonas nitroreducens, Pseudomonas nitroreducens HBP1

9305879

657455

Meyer, A.; Tanner, D.; Schmid, A.; Sargent, D.F.; Kohler, H.P.; Witholt, B.

Crystallization and preliminary X-ray analysis of native and selenomethionine 2-hydroxybiphenyl 3-monooxygenase

Acta Crystallogr. Sect. D

741-743

2003

Pseudomonas nitroreducens

12657798

658323

Held, M.; Schmid, A.; Kohler, H.P.; Suske, W.; Witholt, B.; Wubbolts, M.

An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst

Biotechnol. Bioeng.

641-648

1999

Escherichia coli, Escherichia coli JM101

9951522

744407

Kanteev, M.; Bregman-Cohen, A.; Deri, B.; Shahar, A.; Adir, N.; Fishman, A.

A crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism

Biochim. Biophys. Acta

1854

1906-1913

2015

Pseudomonas nitroreducens (O06647)

26275805

744702

Jensen, C.N.; Mielke, T.; Farrugia, J.E.; Frank, A.; Man, H.; Hart, S.; Turkenburg, J.P.; Grogan, G.

Structures of the Apo and FAD-bound forms of 2-hydroxybiphenyl 3-monooxygenase (HbpA) locate activity hotspots identified by using directed evolution

ChemBioChem

968-976

2015

Pseudomonas nitroreducens (O06647)

25737306

763837

Lazar, J.T.; Shuvalova, L.; Rosas-Lemus, M.; Kiryukhina, O.; Satchell, K.J.F.; Minasov, G.

Structural comparison of p-hydroxybenzoate hydroxylase (PobA) from Pseudomonas putida with PobA from other Pseudomonas spp. and other monooxygenases

Acta Crystallogr. F Struct. Biol. Commun.

507-514

2019

Pseudomonas nitroreducens (O06647), Pseudomonas nitroreducens

31282871

763846

Lazar, J.T.; Shuvalova, L.; Rosas-Lemus, M.; Kiryukhina, O.; Satchell, K.J.F.; Minasov, G.

Structural comparison of p-hydroxybenzoate hydroxylase (PobA) from Pseudomonas putida with PobA from other Pseudomonas spp. and other monooxygenases

Acta Crystallogr. Sect. F

507-514

2019

Pseudomonas nitroreducens (O06647), Pseudomonas nitroreducens

31282871

764475

Bregman-Cohen, A.; Deri, B.; Maimon, S.; Pazy, Y.; Fishman, A.

Altering 2-hydroxybiphenyl 3-monooxygenase regioselectivity by protein engineering for the production of a new antioxidant

ChemBioChem

583-590

2018

Pseudomonas nitroreducens (O06647)

29297973