1.14.13.81: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

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Reaction

Synonyms

AcsF, AcsF protein, aerobic Mg-protoporphyrin IX monomethyl ester cyclase, ATZIP protein, BChE, BNZIP protein, CHL27, CHL27 protein, CRD1, Crd1 protein, CSZIP protein, Cth1 protein, CycI, cyclase, magnesium protoporphyrin IX monomethyl ester, LCAA, LOC_Os01g17170, magnesium protoporphyrin IX monomethyl ester cyclase, magnesium protoporphyrin monomethylester oxidative cyclase, magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, magnesium-protoporphyrin IX monomethyl ester cyclase, magnesium-protoporphyrin-IX monomethyl ester cyclase, magnesium-protoporphyrin-IX monomethyl ester oxidative cyclase, Mg protoporphyrin monomethylester cyclase, Mg-protoporphyrin IX methyl ester cyclase, Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, Mg-protoporphyrin IX monomethyl ester cyclase, Mg-protoporphyrin IX monomethylester cyclase system, Mg-protoporphyrin monomethylester cyclase, MgPME cyclase, MgPME oxidative cyclase, MPE cyclase, MPE cyclase system, MPE-cyclase, MPEC, MPEC gene product, MPME cyclase, NTZIP protein, O2-dependent Mg-protoporphyrin monomethyl ester cyclase, OsCRD1, OSZIP protein, oxygen-dependent Mg-protoporphyrin IX monomethylester cyclase system, oxygen-independent Mg-protoporphyrin IX monomethylester cyclase system, PeMPEC, PNZIP protein, RDZIP protein, rice copper response defect 1, SBZIP protein, Sll1214, SOZIP protein, TAZIP protein, TRZIP protein, XanL, Xantha-l, YSL1

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.81 magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

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Results	in table
13	Activating Compound
3171	AA Sequence
1	Application
1	CAS Registry Number
14	Cloned(Commentary)
33	Cofactor
7	Expression
109	General Information
26	Inhibitors
2	kcat/KM [mM/s]
3	KM Value [mM]
34	Localization
12	Metals/Ions
6	Molecular Weight [Da]
107	Natural Substrates/ Products (Substrates)
114	Organism
5	Pathway
3	pH Optimum
1	pI Value
14	Protein Variants
5	Purification (Commentary)
6	Reaction
4	Reaction Type
107	Reference
2	Renatured (Commentary)
43	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
119	Substrates and Products (Substrate)
21	Subunits
258	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.81 - magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry

Chloroflexus aurantiacus

A9WIR9

698617

partial

Synechocystis sp.

-

636329

partial, soluble fraction

Cucumis sativus

-

636330

partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants

Hordeum vulgare

Q5EFU4

676791

single-subunit O2-dependent cyclase AcsF, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and gel filtration

Rubrivivax gelatinosus

P0DJN9

765462