1.14.13.83: precorrin-3B synthase
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For detailed information about precorrin-3B synthase, go to the full flat file.
Reaction
Synonyms
Bmei0715, CobG, CobZ, precorrin-3A monooxygenase, precorrin-3X synthase
ECTree
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Cloned
Cloned on EC 1.14.13.83 - precorrin-3B synthase
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Brucella melitensis cobG is PCR-amplified, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside
gene cobG heterologously expressed in Escherichia coli
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overexpressed in recombinant strains of Escherichia coli
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Pseudomonas denitrificans cobG is PCR-amplified in plasmid pCR427 and cloned into pET14b, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside
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