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1.14.13.83: precorrin-3B synthase

This is an abbreviated version!
For detailed information about precorrin-3B synthase, go to the full flat file.

Reaction

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precorrin-3A
+
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NADH
+
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H+
+
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O2
=
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precorrin-3B
+
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NAD+
+
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H2O

Synonyms

Bmei0715, CobG, CobZ, precorrin-3A monooxygenase, precorrin-3X synthase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                EC 1.14.13.831.14.13.83 precorrin-3B synthase

Cloned

Cloned on EC 1.14.13.83 - precorrin-3B synthase

for references in articles please use BRENDA:EC1.14.13.83

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Brucella melitensis cobG is PCR-amplified, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside
expressed in Escherichia coli BL21DE3
-
gene cobG heterologously expressed in Escherichia coli
-
genetic and sequence analysis
-
overexpressed in recombinant strains of Escherichia coli
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Pseudomonas denitrificans cobG is PCR-amplified in plasmid pCR427 and cloned into pET14b, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside
-