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1.14.13.84: 4-hydroxyacetophenone monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyacetophenone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.84

Reaction

(4-hydroxyphenyl)ethan-1-one
+
NADPH
 +
H+
 +
O2
=
4-hydroxyphenyl acetate
 +
NADP+
 +
H2O

Synonyms

4-hydroxyacetophenone monooxygenase, arylketone monooxygenase, Baeyer-Villiger monooxygenase, hAPA, HAPMO, More, oxygenase, 4-hydroxyacetophenone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.84 4-hydroxyacetophenone monooxygenase

Cloned

Cloned on EC 1.14.13.84 - 4-hydroxyacetophenone monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli TOP10
-
gene hapE, expression of wild-type and mutant enzymes in Escherichia coli
-
gene hapE, overexpression in Escherichia coli
-
gne hapA, encoded in the hapCDEFGHIBA gene cluster, DNA and amino acid sequence determination and analysis
hapE gene, expression in Escherichia coli TOP10
-
hapE, expression in Escherichia coli BL21(DE3)pLysS, sequence analysis
overexpressed in Escherichia coli
-
overexpression of recombinant enzyme in Escherichia coli BL21(DE3)pLysS
PCR-amplification, expression in Escherichia coli DH5alpha, JM109, BL21 (DE3), and Rosetta