1.14.13.84: 4-hydroxyacetophenone monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyacetophenone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.84

Reaction

(4-hydroxyphenyl)ethan-1-one
+
NADPH
+
H+
+
O2
=
4-hydroxyphenyl acetate
+
NADP+
+
H2O

Synonyms

4-hydroxyacetophenone monooxygenase, arylketone monooxygenase, Baeyer-Villiger monooxygenase, hAPA, HAPMO, More, oxygenase, 4-hydroxyacetophenone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.84 4-hydroxyacetophenone monooxygenase

Purification

Purification on EC 1.14.13.84 - 4-hydroxyacetophenone monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpressing Escherichia coli cells are centrifuged, washed, disrupted with a French pressure cell, centrifuged, supernatant (crude extract) purified to homogeneity in 2 chromatographic steps with Q-Sepharose and Reactive Red
recombinant HAPMO
-
wild-type and mutant HAPMO
-
wild-type, 25fold, and recombinant HAPMO