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1.14.14.1: unspecific monooxygenase

This is an abbreviated version!
For detailed information about unspecific monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.1

Reaction

RH
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
ROH
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

3AH15, 6 beta-hydroxylase, 6-beta-testosterone hydroxylase, 7-alkoxycoumarin O-dealkylase, 7-ethoxycoumarin-O-deethylase, 7-ethoxyresorufin-O-deethylase, AA omega-hydroxylase, Aldehyde oxygenase, Arachidonic acid epoxygenase, aromatase, aryl hydrocarbon hydroxylase, aryl-4-monooxygenase, BG04_163, BM3, BPH, Brain aromatase, class IV cytochrome P450 monooxygenase, clavine oxidase, CLOA, Clone PF26, Clone PF3/46, Coumarin 7-hydroxylase, CP2D6, Cyp, CYP monooxygenase, CYP102, CYP102 monooxygenase, CYP1027H1, CYP102A1, CYP102A2, CYP102A3, CYP102A7, CYP102B1, CYP106, CYP107, CYP1074A2, CYP109, CYP116B3, CYP116B4, CYP116B46, CYP134, CYP150A20, CYP152, CYP154H1, CYP19, CYP197, CYP1A, CYP1A1, CYP1A2, CYP1A3, CYP1B1, CYP24A1, CYP27A1, CYP28A5, CYP2A3, CYP2A6, CYP2B, CYP2B4, CYP2B6, CYP2C11, CYP2C19, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP305A1, CYP3A, CYP3A1, CYP3A4, CYP3A5, CYP3A7, CYP4, CYP4502F4, CYP4A, CYP4A4, CYP4A6, CYP4A7, CYP4AA1, CYP4E2, CYP4F, CYP4F2, CYP4F3A, CYP4F3B, CYP5035A2, CYP5035A3, CYP5035A5, CYP5035C1, CYP5036A1, CYP5036A3, CYP5037B2, CYP505D1, CYP505D2, CYP505D3, CYP505D4, CYP51, CYP512B5, CYP512H1, CYP5136A2, CYP5136A3, CYP5136A5, CYP5139A1, CYP5141A1, CYP5141D1, CYP5142A3, CYP5142C1, CYP5144A3, CYP5144A7, CYP5144A9, CYP5144C7, CYP5144D4, CYP5145A3, CYP5147A1, CYP5150B1, CYP53A15, CYP53C2, CYP53D2, CYP5A1, CYP63A2, CYP6B1, CYP6B1v1, CYP6B1V1/CYP6B1V2/ CYP6B1V3, CYP6B3V1/CYP6B3V2, CYP6B4V1/CYP6B4V2, CYP6B5V1, CYP714D1, CYP82E2, CYP82E3, CYP82E4v1, CYP82E4v2, CYP8A1, CYP9F2, CYPIA1, CYPIA2, CYPIA4, CYPIA5, CYPIB1, CYPIIA1, CYPIIA10, CYPIIA11, CYPIIA12, CYPIIA13, CYPIIA2, CYPIIA3, CYPIIA4, CYPIIA5, CYPIIA6, CYPIIA7, CYPIIA8, CYPIIA9, CYPIIB1, CYPIIB10, CYPIIB11, CYPIIB12, CYPIIB19, CYPIIB2, CYPIIB20, CYPIIB3, CYPIIB4, CYPIIB5, CYPIIB6, CYPIIB9, CYPIIC1, CYPIIC10, CYPIIC11, CYPIIC12, CYPIIC13, CYPIIC14, CYPIIC15, CYPIIC16, CYPIIC17, CYPIIC18, CYPIIC19, CYPIIC2, CYPIIC20, CYPIIC21, CYPIIC22, CYPIIC23, CYPIIC24, CYPIIC25, CYPIIC26, CYPIIC27, CYPIIC28, CYPIIC29, CYPIIC3, CYPIIC30, CYPIIC31, CYPIIC37, CYPIIC38, CYPIIC39, CYPIIC4, CYPIIC40, CYPIIC41, CYPIIC42, CYPIIC5, CYPIIC6, CYPIIC7, CYPIIC8, CYPIIC9, CYPIID1, CYPIID10, CYPIID11, CYPIID14, CYPIID15, CYPIID16, CYPIID17, CYPIID18, CYPIID19, CYPIID2, CYPIID3, CYPIID4, CYPIID5, CYPIID6, CYPIID9, CYPIIE1, CYPIIF1, CYPIIF3, CYPIIF4, CYPIIG1, CYPIIH1, CYPIIH2, CYPIIIA1, CYPIIIA10, CYPIIIA11, CYPIIIA12, CYPIIIA13, CYPIIIA14, CYPIIIA15, CYPIIIA16, CYPIIIA17, CYPIIIA18, CYPIIIA19, CYPIIIA2, CYPIIIA21, CYPIIIA24, CYPIIIA25, CYPIIIA27, CYPIIIA28, CYPIIIA29, CYPIIIA3, CYPIIIA30, CYPIIIA31, CYPIIIA5, CYPIIIA6, CYPIIIA7, CYPIIIA8, CYPIIIA9, CYPIIJ1, CYPIIJ2, CYPIIJ3, CYPIIJ5, CYPIIJ6, CYPIIK1, CYPIIK3, CYPIIK4, CYPIIL1, CYPIIM1, CYPIVA4, CYPIVA8, CYPIVB1, CYPIVC1, CYPIVF1, CYPIVF11, CYPIVF12, CYPIVF4, CYPIVF5, CYPIVF6, CYPIVF8, CYPVIA1, CYPVIB1, CYPVIB2, CYPVIB4, CYPVIB5, CYPVIB6, CYPVIB7, CYPXIX, CYPXIXA1, CYPXIXA2, CYPXIXA3, Cyt P450, cytochrome P-450 4 enzyme, cytochrome P-450 BM3, cytochrome P-450 monooxygenase, cytochrome P450 2B4, cytochrome P450 3A, cytochrome P450 3A4, cytochrome P450 aromatase, cytochrome P450 BM3, cytochrome P450 monooxygenase, cytochrome P450 monooxygenase 116B3, cytochrome P450 monooxygenase 2A6, cytochrome P450 monooxygenase 2C8, cytochrome P450 monooxygenase 2C9, cytochrome P450 monooxygenase 3A4, cytochrome P450 monooxygenase pc-2, cytochrome P450 monooxygenase pc-4, cytochrome P450 monooxygenase pc-5, cytochrome P450 monooxygenase pc-6, cytochrome P450 monooxygenase PC-foxy1, cytochrome P450 oxidoreductase, cytochrome P450 reductase, Cytochrome P450-D2, cytochrome P450-dependent monooxygenase, cytochrome P450-dependent monooxygenase 1A2, cytochrome P450-monooxygenase, cytochrome-P450 hydroxylase, DAH1, DAH2, Debrisoquine 4-hydroxylase, EC 1.14.1.1, EC 1.14.14.2, EC 1.14.99.8, EC 1.99.1.1, ECOD, Ema, EROD, Estrogen synthetase, EUI, fatty acid hydroxylase, flavocytochrome P450BM-3, flavoprotein monooxygenase, flavoprotein-linked monooxygenase, FMO3, FraEuI1c_1415, FraEuI1c_2494, FraEuI1c_5334, GA 16a,17-epoxidase, GA-deactivating enzyme, Hepatic cytochrome P-450MC1, HLp, HMPREF1624_01477, IIA3, Isozyme 3A, Laurate omega-1 hydroxylase, Lauric acid omega-6-hydroxylase, liver cytochrome P450-dependent monooxygenase, LMC1, Mephenytoin 4-hydroxylase, MFO, microsomal monooxygenase, microsomal P-450, mixed function oxygenase, monooxygenase 3, monooxygenase P450 BM-3, More, N-demethylase, nicotine oxidase, O-demethylase, OLF2, Olfactive, Os05g0482400 protein, Ovarian aromatase, oxygenase, flavoprotein-linked mono-, P(3)450, P-448, P-450 PHPAH1, P-450(M-1), P-450-MK2, P-450AROM, P-450IB, P-450IIIAM1, P-450MC, P-450MP, P-450UT, P1-88, P24, P450, P450 17-alpha, P450 19A1, P450 1A1, P450 1A2, P450 1B1, P450 2A6, P450 2B6, P450 2C19, P450 2C9, P450 2D-29/2D-35, P450 2D6, P450 2E1, P450 2J2, P450 3A4, P450 4A11, P450 4F2, P450 BM3, P450 CM3A-10, P450 DUT2, P450 FA, P450 FI, P450 form 3B, P450 form HP1, P450 HSM1, P450 HSM2, P450 HSM3, P450 HSM4, P450 IIB1, P450 IIC2, P450 LM4, P450 LM6, P450 LMC2, P450 MD, P450 monooxygenase, P450 MP-12/MP-20, P450 P49, P450 PB1, P450 PB4, P450 PBC1, P450 PBC2, P450 PBC3, P450 PBC4, P450 PCHP3, P450 PCHP7, P450 TCDDAA, P450 TCDDAHH, P450 type B2, P450 types B0 and B1, P450(I), P450-11A, P450-15-alpha, P450-15-COH, P450-16-alpha, P450-254C, P450-3C, P450-6B/29C, P450-A3, P450-AFB, P450-ALC, P450-BM3, P450-CMF1A, P450-CMF1B, P450-CMF2, P450-CMF3, P450-DB1, P450-DB2, P450-DB3, P450-DB4, P450-DB5, P450-HFLA, P450-HP, P450-IIA10, P450-IIA11, P450-IIA3.1, P450-IIA3.2, P450-IIA4, P450-KP1, P450-LM2, P450-MC1, P450-MC4, P450-MK1, P450-MKJ1, P450-MKMP13, P450-MKNF2, P450-NMB, P450-OLF1, P450-OLF3, P450-P1, P450-P2/P450-P3, P450-P3, P450-PB1 and P450-PB2, P450-PCN1, P450-PCN2, P450-PCN3, P450-PM4, P450-PP1, P450-PROS2, P4501A1, P450arom, P450cam, P450CB, P450CMEF, P450E, P450EF, P450F, P450H, P450I, P450IIC5, P450MT2, P450RAP, P450RLM6, P450s 3A, P450SMO, P52, PB15, PHP2, PHP3, PikC, PikC hydroxylase, Progesterone 21-hydroxylase, Prostaglandin omega-hydroxylase, PTF1, PTF2, S-mephenytoin 4-hydroxylase, Sam5, sertraline N-demethylase, SIAM614_30676, Steroid hormones 7-alpha-hydroxylase, Testosterone 15-alpha-hydroxylase, Testosterone 16-alpha hydroxylase, Testosterone 6-beta-hydroxylase, Testosterone 7-alpha-hydroxylase, xenobiotic monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.1 unspecific monooxygenase

Cloned

Cloned on EC 1.14.14.1 - unspecific monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus-infected insect cells expressing single c-DNA-derived CYPs
both isozymes CYP52A13, CYP52A17
-
chimeric oxygenase, in which the P450cam domain is fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784. Plasmids containing the P450cam-RhFRed gene (L1-L7) transformed into Escherichia coli BL21-DE3cells
-
coexpression of CYP1A2 with the Y459H and V492E mutant POR alleles in tBTC1A2_POR cell-models
-
CYP82E2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants
Q38Q84, Q38Q85, Q38Q86, Q38Q87
CYP82E3, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants
Q38Q84, Q38Q85, Q38Q86, Q38Q87
CYP82E4v1, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants
Q38Q84, Q38Q85, Q38Q86, Q38Q87
CYP82E4v2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11
Q38Q84, Q38Q85, Q38Q86, Q38Q87
DNA and amino acid sequence determination and analysis, and genetic structure and organization analysis of several genes, classification of 12 families and 23 subfamilies, two representative multigene P450 families CYP63 and CYP505, i.e. P450foxy, complex structural features of the P450 genes in this genome, including microexons, of 2-10 amino acids, and frequent small introns of 45-55 base pairs, tandem gene clusters, alternative splicing and functional diversity, phylogenetic analysis of the gene families, detailed overview
DNA and amino acid sequence determination and analysis, and genetic structure and organization analysis, phylogenetic analysis, overview
DNA sequence and genomic organization determination and analysis, usage of directional genome walking method, overexpression of His6-tagged enzyme in Escherichia coli starin BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli C43(DE3) cells
-
expressed in Escherichia coli strain C43(DE3)
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain KM71H
-
expressed in Saccharomyces cerevisiae
expression analysis of human CYPS, overvuew, expression in Escherichia coli without the N-terminal leader sequence
-
expression anbalysis at restricted feeding versus ad libitum
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3) using the pET281 expression system
expression in Escherichia coli in fed-batch fermentation
expression in HepG2 cells
expression in V79 Chinese hamster fibroblasts and Hep-G2 cells, coexpression with N(O)-acetyltransferase or sulfotransferase 1A1-1
-
expression of mutant H226Y in Escherichia coli
-
expression of recombinant human P4502B6 and yeast reductase fused enzyme in transgenic tobacco plants, integration into the tobacco genome, functional expression of CYP1A1 and CYP1A1-yeast reductase fused enzyme in transgenic potato plants, genetic transformation is mediated by Agrobacterium tumefaciens
-
expression of wild-type and mutant enzymes in Escherichia coli NovaBlue(DE3)
-
exprression in Escherichia coli
functional expression of the fused enzyme between rat CYP1A1 and rat or yeast reductase in transgenic tobacco and potato plants mainly in microsomes
-
gene cloA, localization in the ergot alkaloid gene cluster, DNA and amino acid sequence determination and analysis, complementation of enzyme-deficient mutant DELTAP450-1
-
gene crtS, DNA and amino acid sequence determination of different strains
-
gene eui, i.e. elongated uppermost internode, map-based cloning, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of EUI in Saccharomyces cerevisiae strain WAT11 microsomes, expression of a chimeric EUI–GFP in onion epidermic cells with localization in the cytoplasm on the surface of the endoplamic reticulum
gene family Ema, DNA and amino acid sequence determination and analysis
-
gene family ema, DNA and amino acid sequence determination and analysis, overexpression of His-tagged ema1 and ema16 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli and Streptomyces lividans
-
gene pikC, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
high level and functional expression of P450SMO in Escherichia coli strain BL21, method optimization and evaluation, overview
-
homologous peptide fragments of terpene omega-hydroxylase CYP4C7 from Diploptera punctata inserted into CYP102A1
into pET21a vector, amplified in Escherichia coli DH5alpha cells and expressed under control of the T7 promoter in Escherichia coli BL21 Star (DE3)
into pET21d vector, amplified in Escherichia coli DH5alpha cells and expressed under control of the T7 promoter in Escherichia coli BL21 Star (DE3)
into the pET28a (+) vector and expressed in Escherichia coli BL21 (DE3)
-
microsomes derived from baculovirus infected insect cells expressing CYP1A2
microsomes derived from baculovirus infected insect cells expressing CYP2B6
microsomes derived from baculovirus infected insect cells expressing CYP2C8
microsomes derived from baculovirus infected insect cells expressing CYP2C9, CYP2C19, CYP2E1, CYP3A4, or CYP19
microsomes derived from baculovirus infected insect cells expressing CYP2D6
microsomes derived from baculovirus infected insect cells expressing CYP3A5
microsomes derived from baculovirus infected insect cells expressing CYP3A7
overexpression in Pichia pastoris. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition
plasmid propagation in Escherichia coli DH5alpha, heterologous expression of CYP102A1, CYP102A2, CYP102A3 and CYP102A7 from vector pET 28a(+) in Escherichia coli BL21 (DE3)
-
recombinant cDNA P450s coexpressed with cytochrome P450 reductase in insect cell microsomes
-
recombinant expression in Escherichia coli
-
V79-hCYP2E1 (constructed by transfection of CYP2E1-expressing vector pSV together with vector pSV2neo containing a G418-selective marker into V79-Mz cells) and SULT1A1 expression vector introduced into this cell line
-