site-directed mutagenesis. The introduction of a smaller amino acid at position 87 results in a more active monooxygenase and a different product profile for the oxidation of substrate (-)-alpha-pinene.
site-directed mutagenesis. MD simulations of the double mutant A74G L188Q (GQ) show that the substrate is blocked from accessing the heme oxygen by the side chain of the F87, when it adopts the conformation found in the X-ray structure.
site-directed mutagenesis, the mutant exhibits an altered regioselectivity and substrate specificity compared with wild-type, it has lower tolerance toward DMSO
the mutant enzyme retains significant hydroxylase activity towards saturated fatty acids and shows much increased activity towards C12-C16 alkyl trimethylammonium compounds
site-directed mutagenesis. The introduction of a smaller amino acid at position 87 results in a more active monooxygenase and a different product profile for the oxidation of substrate (-)-alpha-pinene.
construction of a disrution-inactivated, enzyme-deficient mutant DELTAP450-1, that accumulates agroclavine, elymoclavine, and chanoclavine, but not ergopeptines, feeding experiments
construction of a disrution-inactivated, enzyme-deficient mutant DELTAP450-1, that accumulates agroclavine, elymoclavine, and chanoclavine, but not ergopeptines, feeding experiments
genetic variations, substantial effects of single nucleotide polymorphisms, e.g. CYP2D6 and CYP2C19 SNPs show large e.ects on metabolism of debrisoquine and (S)-mephenytoin, respectively, overview
transgenic tobacco plants expressing rat CYP1A1 and its fused enzyme with yeast reductase showed tolerance to the herbicide chlorotoluron, some of them are tolerant to the herbicides diuron and atrazine, the transgenic plants also show increased activity against 7-ethoxycoumarin, overview
construction of the recessive elongated uppermost internode tall mutant, which is morphologically normal until its final internode elongates drastically at the heading stage, the stage-specific developmental effect of the eui mutation is used in the breeding of hybrid rice to improve the performance of heading in male sterile cultivars, the eui mutant accumulates exceptionally large amounts of biologically active gibberellins in the uppermost internode, eui mutant plants and Eui-overexpressing transgenic plants show tall and dwarfed phenotypes, and fail to produce seeds, overview
construction of the recessive elongated uppermost internode tall mutant, which is morphologically normal until its final internode elongates drastically at the heading stage, the stage-specific developmental effect of the eui mutation is used in the breeding of hybrid rice to improve the performance of heading in male sterile cultivars, the eui mutant accumulates exceptionally large amounts of biologically active gibberellins in the uppermost internode, eui mutant plants and Eui-overexpressing transgenic plants show tall and dwarfed phenotypes, and fail to produce seeds, overview
construction and analysis of insertion mutant strains defective in biosynthesis of astaxanthin, the mutants are selected by accumulation of astaxanthin precursors
construction and analysis of insertion mutant strains defective in biosynthesis of astaxanthin, the mutants are selected by accumulation of astaxanthin precursors
the herbicide chlorotoluron delayed the germination and development of Orobanche ramosa seeds in case of use of transgenic plants tolerant to the herbicide
the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications
the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications
mutants of enzyme P450-BM3 evolved by directed evolution are excellent catalysts in the CH-activating oxidative hydroxylation of 1-tetralone derivatives and of indanone, with unusually high regio- and enantioselectivity
mutants of enzyme P450-BM3 evolved by directed evolution are excellent catalysts in the CH-activating oxidative hydroxylation of 1-tetralone derivatives and of indanone, with unusually high regio- and enantioselectivity
construction of a fused enzyme between rat CYP1A1 and rat reductase on microsomes in the recombinant yeast cells, which showed enhanced specific activity as compared with the original enzyme system
site-specific mutagenesis and screening for a gain of function mutant of CYP116B3, dealkylation activity of 12 variants chosen from the primary screening, overview. Identification of a mutant 74H10 that shows a 240fold increased deethylation activity toward 7-ethoxycoumarin and a 10fold increased demethylation activity toward 7-methoxycoumarin
transgenic potato plants expressing CYP1A1 and its fused enzyme with yeast reductase showed tolerance to the herbicide chlorotoluron, some of them are tolerant to the herbicides diuron and atrazine, the transgenic plants also show increased activity against 7-ethoxycoumarin, overview