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1.14.14.19: steroid 17alpha-monooxygenase

This is an abbreviated version!
For detailed information about steroid 17alpha-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.19

Reaction

a C21-steroid
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 17alpha-hydroxy-C21-steroid
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

17 alpha-hydroxylase/C17,20-lyase, 17,20-lyase, 17-alpha-hydroxylase/C17-20 lyase, 17-hydroxylase/17,20-lyase, 17-hydroxylase/C17,20-lyase, 17alphahydroxylase, 17alpha-hydroxylase, 17alpha-hydroxylase 17,20 lyase, 17alpha-hydroxylase-17,20-lyase, 17alpha-hydroxylase-C17,20 lyase, 17alpha-hydroxylase-C17,20-lyase, 17alpha-hydroxylase/17,20 lyase, 17alpha-hydroxylase/17,20-lyase, 17alpha-hydroxylase/C(17,20)-lyase, 17alpha-OHase, 17OHD, CYP 17, CYP17, CYP17A1, CYPXVII, cytochrome P-450 (P45017alpha,lyase), cytochrome P-45017alpha, cytochrome P450 17, cytochrome P450 17A1, cytochrome P450 17A2, cytochrome P450 17alpha-hydroxylase, cytochrome P450 17alpha-hydroxylase-17,20-lyase, cytochrome P450 17alpha-hydroxylase/17,20 lyase, cytochrome P450 17alpha-hydroxylase/17,20-lyase, cytochrome p450 17alpha-hydroxylase/C(17,20)-lyase, cytochrome P450 17alpha-hydroxylase/C17,20-lyase, cytochrome P450 17alpha-hydroxylase/c17-20 lyase, cytochrome P45017alpha, cytochrome P450c17, cytochrome P450c17alpha, cytochromeP450 17alpha-hydroxylase/C17-20 lyase, EC 1.14.1.7, EC 1.14.99.9, EC 1.99.1.9, P450 17, P450 17A1, P450(17alpha), P450-17alpha, P450-C17, P45017alpha, P450c17, P450c17-I, Steroid 17-alpha-hydroxylase/17,20 lyase, steroid 17alpha-hydroxylase, steroidogenic cytochrome P450 17A1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.19 steroid 17alpha-monooxygenase

Cloned

Cloned on EC 1.14.14.19 - steroid 17alpha-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of CYP17 and NADPH-P450 reductase in Escherichia coli, expression of the enzyme in insect cells via baculovirus transfection
-
CYP17, expression in Escherichia coli, co-expression with NADPH-P450 reductase
-
CYP17, real-time RT-PCR expression analysis
CYP17A1, DNA and amino acid sequence determination and analysis, expression of mutant R239Q in HEK-293 cells
-
DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, genotyping
-
DNA and amino acid sequence determination and analysis, CYP17 genotyping, realtime PCR expression analysis
expressed in Escherichia coli
-
expressed in Escherichia coli BL21-Gold (DE3) cells
expressed in Escherichia coli JM109 cells
expression in Cos-1 cells
-
expression in Escherichia coli cells
-
expression in Escherichia coli JM109, human gene with an (His)4-tail
expression in Escherichia coli XL1
-
expression in Escherichia coli, human gene with an (His)4-tail
-
expression in HEK-293T cell
expression in monkey kidney COS-1 cells
expression in Saccharomyces cerevisiae strain AH22, fused with yeast reductase, greater 17alpha-hydroxylase activity
-
expression in Saccharomyces cerevisiae strain W303B of the K89N-mutant
expression of His-tagged enzyme in Escherichia coli strain JM109, coexpression with molecular chaperones GroES and GroEL
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109, co-expression of P450c17 and p38 in COS-1 cells
expression of wild-type and mutant C-terminally Bis-tagged enzymes lacking the N-terminal transmembrane helix in Escherichia coli strain JM109
expression of wild-type and mutant enzymes in yeast microsomes, expression of modified CYP17A1 in Escherichia coli strain JM109
functional expression of cytochrome P45017alpha in Pichia pastoris strain GS115, subcloning in Escherichia coli strain JM109
-
gene CYP17, coexpression in Escherichia coli with human NADPH-P450 reductase
-
gene CYP17, expression analysis
-
gene CYp17, expression in Escherichia coli, coexpression with human cytochrome P450 reductase
-
gene CYP17, expression in HeLa cells
-
gene CYP17A1, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, overview
-
gene CYP17A1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semi-quantitative RT-PCR analysis of spatial expression
gene CYP17A1, genotyping
-
gene p450c17, cDNA from ovary, DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression in COS-7 cells, overexpression in Escherichia coli
gene P450c17-I, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semiquantitative RT-PCR enzyme expression analysis
gene P450c17-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semiquantitative RT-PCR enzyme expression analysis
genes P450c17-I and P450c17-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, and quantitative real-time PCR expression analysis
-
recombinant expression in Escherichia coli
-
recombinant expression in yeast microsomes
the P450c17 domain of the coding sequence is connected to a truncated form of rat NADPH-P450 reductase by a linker sequence encoding Ser-Thr, functional expression of fusion proteins in Escherichia coli