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1.14.14.20: phenol 2-monooxygenase (FADH2)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (FADH2), go to the full flat file.

Word Map on EC 1.14.14.20

Reaction

phenol
+
FADH2
+
O2
=
catechol
+
FAD
+
H2O

Synonyms

FAD reductase, flavin reductase PheA2, PheA, PheA1, PheA2, phenol hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.20 phenol 2-monooxygenase (FADH2)

Storage Stability

Storage Stability on EC 1.14.14.20 - phenol 2-monooxygenase (FADH2)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, no significant loss of activity of the flavin reductase component PheA2 after 4 months
-70°C, purified recombinant PheA1 protein is not very stable when stored at -70°C because it forms aggregates after thawing
-70°C, purified recombinant PheA2 protein is stable for 4 months when stored at -70°C
4°C, purified recombinant PheA1 is stable when stored as a protein precipitate in 80% ammonium sulfate at 4°C