Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.3: bacterial luciferase

This is an abbreviated version!
For detailed information about bacterial luciferase, go to the full flat file.

Word Map on EC 1.14.14.3

Reaction

a long-chain aldehyde
+
FMNH2
 +
O2
=
a long-chain fatty acid
 +
FMN
 +
H2O
 +
hnu

Synonyms

4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.3 bacterial luciferase

Cloned

Cloned on EC 1.14.14.3 - bacterial luciferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified from the pJHD500 plasmid and ligated into a pET21b vector, expressed from pZCH2 in an Escherichia coli BL21 (lambdaDE3) cell line after growth to an OD600 of 0.5
coexpression of luciferase and cytochrome P-450
-
expressed in Escherichia coli BL21(DE3) and HEK-293T cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli from pJHD500, ligated into a pET21b vector. Luciferase subcloned from pZCH2 into a pASKIBA-3c vector with the restriction sites XbaI and XhoI. The resulting luciferase containing a strep-II tag on the C terminus of the beta-subunit (pZCB4) expressed
-
expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme)
-
expressed in NIH-3T3 cells
-
expressed in Pseudomonas putida mt-2
-
expression analysis, cloning into expression vector pPL2lux useable as a reporter system based on the luciferase activity of the enzyme, overview
-
expression in Bacillus subtilis and in Escherichia coli
expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively
expression in Escherichia coli
expression in Escherichia coli strain JM101
-
expression in Pseudomonas putida
-
expression of fused luxA and luxB genes and also luxF gene in Escherichia coli and Nicotiana plumbaginifolia
-
expression of fused luxA and luxB genes in Escherichia coli
expression of fused luxA and luxB genes in Saccharomyces cerevisiae, Bacillus subtilis, plant cells, plasmid expression vector and in Escherichia coli
-
expression of luxA gene in Escherichia coli
-
expression of seperated luxA and luxB gene in Escherichia coli JM109
-
expression of the enzyme in Mycobacterium tuberculosis under control of the inducible/repressible promotor of the alanine dehydrogenase from Mycobacterium tuberculosis strain H37Rv, usage of a mycobacterial-Escherichia coli shuttle vector
-
expression of wild-type and mutant C106A enzymes in Escherichia coli strain JM101
-
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and randomly generated mutants in Escherichia coli strain BL21
-
gene Gluc, expression in Mycobacterium smegmatis using an hsp60 promoter, subcloning in Escherichia coli strain DH5alpha
-
gene lux, improvement of a tagging system for luciferase by construction of a highly active, constitutive promoter resulting in a 100fold higher recombinant activity compared to native activity
-
gene luxA, expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
gene luxAB, expression of wild-type and mutant enzymes in Escherichia coli strain BL21
-
gene luxAB, functional coexpression in Saccharomyces cerevisiae with NADPH-specific FMN reductase FRP from Vibrio harveyi, subcloning in Escherichia coli
-
genes luxA and luxB, expression under the control of a consensus-type promoter, lacUV5, in Escherichia coli, activity declines abruptly upon entry into the stationary growth phase, while the levels of luciferase proteins remian constant, the phenomenon, termed ADLA, i.e. abrupt decline of luciferase activity, is caused by a decrease in the availability of flavin mononucleotide
-
ligated into pET21-b vector, expressed from pZCH2 in Escherichia coli BL21 (lambdaDE3) cell line
-
luxCDABE operon, genetic organization, overview
overexpression in Escherichia coli
-
overexpression of mutant in XL1 blue MRF' cell line
-
overexpression of wild-type and mutant enzymes in Escherichia coli strain JM101
-
stable expression, using a bicistronic expression vector, of wild type luxA and luxB, WTA/WTB, codon-optimized luxA and wild type luxB, COA/WTB, and codon-optimized versions of both luxA and luxB genes, COA/COB, in HEK-293 cells, expression analysis, method evaluation and optimization, highest bioluminescence by expression of both codon-optimized genes, overview
the bacterial luciferase lux gene cassette consists of five genes, luxCDABE. The lux operon is re-synthesized through a process of multibicistronic, codon-optimization to demonstrate self-directed bioluminescence emission in a mammalian HEK-293 cell line in vitro and in vivo, overview. To overcome the limitations by FMNH2 supply, co-expression of a constitutively expressed flavin reductase gene frp from Vibrio harveyi is performed leading to a 151fold increased increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes
-