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1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.9

Reaction

4-hydroxyphenylacetate
+
FADH2
+
O2
=
3,4-Dihydroxyphenylacetate
+
FAD
+
H2O

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

Molecular Weight

Molecular Weight on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
gel filtration
18679
-
x * 18679, SDS-PAGE, enzyme is needed for activity
18680
-
x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence
19000
-
2 * 19000 small component, 2 * 59000 large component
209000
-
component C2, gel filtration
30750
-
2 * 30750, small component
32000
-
x * 32000, small component, SDS-PAGE
32500
-
2 * 32500, alpha2 oligomeric structure, SDS-PAGE
35000
x * 35000, C1 component, SDS-PAGE, x * 47000, C2 component, SDS-PAGE
38500
-
1 * 38500, large component
47000
x * 35000, C1 component, SDS-PAGE, x * 47000, C2 component, SDS-PAGE
50000
-
homotetramer, 4 * 50000, large component, SDS-PAGE
56000
x * 56269, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE
56269
x * 56269, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE
58500
-
x * 58500, SDS-PAGE
58781
59000
65000
-
gel filtration
73000
-
component C1, gel filtration
91000
-
gel filtration