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1.14.15.3: alkane 1-monooxygenase

This is an abbreviated version!
For detailed information about alkane 1-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.3

Reaction

Octane
+
reduced rubredoxin
 +
O2
 + 2 H+ =
1-Octanol
 +
oxidized rubredoxin
 +
H2O

Synonyms

1-hydroxylase, alkane 1-hydrolase, alkane hydroxylase, alkane mono-oxygenase, alkane monooxygenase, alkane omega-hydroxylase, alkane-1-monooxygenase 1, alkane-1-monooxygenase 2, AlkB, AlkB1, AlkB2, AlkW1, AlkW2, AlmA, AlmA-like alkane hydroxylase, BMO, butane monooxygenase, CYP omega hydroxylase, CYP omega-hydroxylase, Cyp153, CYP153 homologue, CYP153A, CYP153D17, CYP4 P450, CYP450 4A omega-hydroxylase, CYP450 omega-hydroxylase, CYP4A, CYP4A10, CYP4A11, CYP4A14, CYP4AII, CYP4d21, CYP86A1, CYP86B1, CYP94A1, CYPA11 P450, CYPIVA1, CYPIVA11, CYPIVA2, CYPIVA3, CYPIVA5, CYPIVA6, CYPIVA7, cytochrome P-450 omega-hydroxylase, cytochrome P450 alkane hydroxylase, cytochrome P450 omega hydroxylase, cytochrome P450-dependent fatty acid omega-hydroxylase, diiron omega-hydroxylase, FA omega-hydroxylase, fatty acid omega-hydrolase, Fatty acid omega-hydroxylase, Gpo1, hexane hydroxylase, Lauric acid omega-hydroxylase, MAH1-1, MAH1-2, male-specific fatty acid omega-hydroxylase, medium-chain alkane hydroxylase, methane monooxygenase, More, omega hydroxylase, omega-hydrolase, omega-hydroxylase, P-450 HK omega, P450-HL-omega, P452, Prm1ABCD, Prm2ABCD, propane monooxygenase, Rieske-type alkane monooxygenase, sex-specific enzyme 1, SXE1, UAM-Ps1, very long chain fatty acid hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.3 alkane 1-monooxygenase

Engineering

Engineering on EC 1.14.15.3 - alkane 1-monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D323E
-
gene CYP4A1
E320A
-
gene CYP4A1
E320A/D323E
-
gene CYP4A1
L69F
-
oxidizes only C10 and C11 alkanes
L69W
-
oxidizes only C10 and C11 alkanes
L69F
-
oxidizes only C10 and C11 alkanes
-
L69W
-
oxidizes only C10 and C11 alkanes
-
H206Y
-
gene CYP4A7
H206Y/S255F
-
gene CYP4A7
R90W
-
gene CYP4A7
R90W/W93S
-
gene CYP4A7
S255F
-
gene CYP4A7
W93S
-
gene CYP4A7
H138A
-
no activity
H142A
-
no activity
H163A
-
~50% of activity
H168A
-
no activity
H172A
-
no activity
H173A
-
no activity
H181A
-
no activity
H308A
-
~30% of activity
H312A
-
no activity
H315A
-
no activity
H316A
-
no activity
H318A
-
~25% of activity
H273A
-
no activity
H273Q
-
no activity
K18A
-
complete loss of activity, mutant protein display an altered secondary structure and decreased melting temperature
K193A
-
5-20% loss of activity
K359A
-
5-20% loss of activity
W55C
-
prefers C11-C13 alkanes as growth substrates
W55S
-
prefers C11-C13 alkanes as growth substrates
H273A
-
no activity
-
H273Q
-
no activity
-
W55C
-
prefers C11-C13 alkanes as growth substrates
-
W55S
-
prefers C11-C13 alkanes as growth substrates
-
additional information