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1.14.15.4: steroid 11beta-monooxygenase

This is an abbreviated version!
For detailed information about steroid 11beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.4

Reaction

A steroid
+ 2 reduced adrenodoxin +
O2
 + 2 H+ =
an 11beta-hydroxysteroid
 + 2 oxidized adrenodoxin +
H2O

Synonyms

11-hydroxylase, 11 beta-hydroxylase, 11-beta hydroxylase, 11-beta-hydroxylase, 11-hydroxylase, 11beta hydroxylase, 11beta-hydroxylase, ALDOS, Aldosterone synthase, Aldosterone-synthesizing enzyme, C450XIB2, CYP11B1, CYP11B2, cyp11beta, CYPXIB, CYPXIB1, CYPXIB2, CYPXIB3, cytochrome 11beta-hydroxylase, cytochrome P-45011-beta, cytochrome P450 11B1, cytochrome P450 11B2, cytochrome P450 11beta-hydroxylase, cytochrome P45011beta, cytochrome P45011beta-hydroxylase, EC 1.14.1.6, EC 1.99.1.7, P-450(11 beta,aldo), P-450Aldo, P-450c11, P-450C18, P-450XIB1, P450 11beta-hydroxylase, P450(11 beta)-DS, P45011beta, P450C11, Steroid 11-beta-hydroxylase, steroid 11-hydroxylase, steroid 11beta-hydroxylase, steroid 11beta-monooxygenase, steroid 11beta/18-hydroxylase, Steroid 18-hydroxylase, steroid-11beta-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.4 steroid 11beta-monooxygenase

Storage Stability

Storage Stability on EC 1.14.15.4 - steroid 11beta-monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, 1 week, without significant loss of activity
-
-20°C, purified enzyme, 24 h, addition of 20% glycerol, 0.5 mM PMSF, 0.5 mM DTT, 95.3% remaining activity
-
-30°C, extract precipitate stable, solution less stable, DEAE fraction unstable
-
-30°C, preserved by Tween 80, conservation of activity
-
-80°C, 20 mM potassium phosphate buffer, pH 7.4, 0.01 mM 11-deoxycorticosterone, 0.1 mM EDTA, 0.1 mM dithiothreitol, 1%, w/v, sodium cholate
-
-80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20
-
-80°C, after ammonium sulfate precipitation, 50 mM potassium phosphate, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.5% sodium cholate, 0.5% soybean lecithin, several months, without loss of activity
-
-80°C, after hydroxyapatite column/FPLC system, 50 mM potassium phosphate, pH 7.4, 0.01% sodium cholate, 0.05% soybean lecithin, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA, without loss of activity
-
-80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA
-
-80°C, purified, 200 mM sodium phosphate buffer, pH 8.0, 0.2 mM 11-deoxycortisol, 20% glycerol, 0.1 mM GSH, 0.1% sodium cholate, several days without loss of activity
-
0°C, preserved by Tween 80, conservation of activity
-
2°C, crude extract loses 50% activity in 24 h
-
4°C, 1 week, without significant loss of activity
-
4°C, 11 days, both activities decrease considerably in a similar way, deoxycorticosterone delays decline
-
4°C, enzyme incorporated into liposomes, stable during 2 weeks
-
4°C, soluble form, loses part of activity in few days, after 2 weeks 1/4 of original activity observed
-
4°C, sonicated mitochondria, stable
-
blood samples, 4°C
-
purified or partially purified enzyme unstable during storage
-