1.2.1.100: 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase
This is an abbreviated version!
For detailed information about 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, go to the full flat file.
Reaction
Synonyms
mlr6739, mlr6793
ECTree
1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.2.1.100 - 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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?
additional information
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enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+ and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH, i.e. reaction of 4-pyridoxic acid dehydrogenase. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate
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additional information
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enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
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additional information
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enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
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?
additional information
?
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enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
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additional information
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enzyme catalyzes with equal facility both the oxidation of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NAD+ to 3-hydroxy-2-methylpyridine-4,5-dicarboxylic acid and the reduction of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NADH to 4-pyridoxic acid, i.e. reaction of 4-pyridoxic acid dehydrogenase. No oxidation of 4-pyridoxic acid is observed
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