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1.2.1.11: aspartate-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about aspartate-semialdehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.11

Reaction

L-aspartate 4-semialdehyde
+
phosphate
 +
NADP+
=
L-4-aspartyl phosphate
 +
NADPH
 +
H+

Synonyms

AFUA_3G06830, ASA dehydrogenase, ASA DH, ASA-DH, ASADH, ASADHD, Asd, ASD enzyme, Asd1, Asd2, AsdA, aspartate beta-semialdehyde dehydrogenase, aspartate semialdehyde dehydrogenase, aspartate-beta-semialdehyde dehydrogenase, aspartate-beta-semialdeyhyde dehydrogenase, aspartate-semialdehyde dehydrogenase, aspartic acid semialdehyde dehydrogenase, aspartic beta-semialdehyde dehydrogenase, aspartic semialdehyde dehydrogenase, aspartyl beta-semialdehyde dehydrogenase, aspartyl semialdehyde dehydrogenase, BDCG_01946, CNA02450, dehydrogenase, aspartate semialdehyde, ecASADH, FtASADH, hiASADH, L-aspartate-beta-semialdehyde dehydrogenase, L-aspartate-beta-semialdehyde:NADP oxidoreductase (phosphorylating), Rv3708c

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.11 aspartate-semialdehyde dehydrogenase

Cloned

Cloned on EC 1.2.1.11 - aspartate-semialdehyde dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and recombinant protein overproduction with Escherichia coli
co-expression with L-lysine-insensitive aspartokinase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase in the parent strain and in an AEC-resistant mutant strain of Lactobacillus plantarum from a shuttle vector, leading to increased L-lysine production, but absolutely requiring overexpression of aspartokinase or dihydrodipicolinate synthase, overview
-
expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli M15 cells
expressed in Escherichia coli, the genome contains two genes for the enzyme
-
expression as His-tag fusion protein in Escherichia coli BL21 (DE3)
-
expression Escherichia coli
expression in Escherichia coli
expression in Escherichia coli Hfr3000
-
expression of His-tagged enzyme in Escherichia coli strain M15
expression of wild-type and mutant enzymes
-
gene AFUA_3G06830, codon-optimized, recombinant expression of C-terminally His-tagged enzyme ASADH in Escherichia coli strain BL21(DE3)
gene asd, cloning from genomic DNA library, DNA sequence determination and analysis, expression in enzyme-deficient auxotrophic Escherichia coli strain CGSC5080
gene asd, construction of a genetic ecASADH library by saturation mutagenesis, recombinant expression of His-tagged wild-type and selected mutants in Escherichia coli strain BL21(DE3)
gene asd, phylogenetic analysis
gene asd, sequence comparisons, recombinant expression of N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene asd, subcloning in Escherichia coli strain JM109, DNA sequence determination and analysis, optimization of functional overexpression in Escherichia coli strain M15 as His-tagged protein
gene BDCG_01946, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli
gene CNA02450, codon-optimized, recombinant expression of C-terminally His-tagged enzyme ASADH in Escherichia coli strain BL21(DE3)
genes are amplified from chromosomal DNA of Lactobacillus plantarum and cloned into the pCR2.1-TOPO vector for sequencing, and subcloned into the pUC18 vector for heterologous expression in Escherichia coli
-
heterologous expression in Escherichia coli
HOM2 gene encodes aspartic semialdehyde dehydrogenase
-
into a pET28a vector for expression in Escherichia coli BL21DE3
-
into the pET41-41a vector for transformation into Rosetta-competent Escherichia coli cells
-
recombinant large scale expression in strain BL21(DE3)