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1.2.1.19: aminobutyraldehyde dehydrogenase

This is an abbreviated version!
For detailed information about aminobutyraldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.19

Reaction

4-aminobutanal
+
NAD+
+
H2O
=
4-aminobutanoate
+
NADH
+
H+

Synonyms

4-aminobutanal dehydrogenase, 4-aminobutyraldehyde dehydrogenase, ABAL dehydrogenase, ABALDH, ALDH10A8, ALDH10A9, AMADH, AMADH1, AMADH2, aminoaldehyde dehydrogenase, BADH, betaine aldehyde dehydrogenase, dehydrogenase, aminobutyraldehyde, EC 1.5.1.35, gadbh, gamma-aminobutanal dehydrogenase, gamma-aminobutyraldehyde dehydroganase, gamma-aminobutyraldehyde dehydrogenase, gamma-guanidinobutyraldehyde dehydrogenase, MdAMADH1, MdAMADH2, More, NAD+-aminoaldehyde dehydrogenase, NAD+-dependent aminoaldehyde dehydrogenase, PatD, PsAMADH 1, PsAMADH 2, SlAMADH1, SlAMADH2, YdcW, ZmAMADH1a, ZmAMADH1b, ZmAMADH2

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.19 aminobutyraldehyde dehydrogenase

Purification

Purification on EC 1.2.1.19 - aminobutyraldehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE Sephacel, hydroxyapatite, 5'-AMP Sepharose 4B, Mono Q, TSK-GEL
-
cobalt- or nickel-charged IDA-Sepharose column chromatography and Resource Q column chromatography
from putrescine-grown cells, using ammonium sulfate fractionation and column chromatography on Sephacryl S-300, DEAE-Sephacel and Blue-Sepharose CL6B
-
from putrescine-grown cells, using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, Sephadex G-200, Hydroxylapatite and Sephadex G-200 in 150 mM NaCl
-
native enzyme 27.22fold from germinated seeds by ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography
recombinant His-tagged enzyme from in Escherichia coli by affinity chromatography
recombinant His-tagged isozyme AMADH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged isozyme AMADH2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
to homogeneity by ammonium sulfate precipitation, anion-exchange chromatography and hydrophobic interaction chromatography
using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, AMP-Sepharose, Sephadex G-200 and NAD+-Sepharose
-
using CM-trisacryl, DEAE-Sephacel, 5'-AMP-Sepharose affinity chromatography, and fast protein liquid chromatography on a mono-Q column
-
using column chromatography on DEAE-cellulose
-
using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue
-
using column chromatography on DEAE-cellulose, hydroxylapatite, Sephacryl S-300 and Phenyl-Superose and isoelectrofocusing
-
using column chromatography on hydroxylapatite, precipitation with ammonium sulfate, column chromatography on Sephacryl S-300, Phenyl-superose, 5'-AMP-Sepharose and hydroxylapatite
-
using protamine, ammonium sulfate, acetone and column chromatography on DEAE-cellulose
-