1.2.1.20: glutarate-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about glutarate-semialdehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.20

Reaction

5-Oxopentanoate
+
NAD+
+
H2O
=
Glutarate
+
NADH
+ 2 H+

Synonyms

davD, davDT davDT, dehydrogenase, glutarate semialdehyde, glutarate dialdehyde dehydrogenase, glutarate semialdehyde dehydrogenase, GSA dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.20 glutarate-semialdehyde dehydrogenase

Cloned

Cloned on EC 1.2.1.20 - glutarate-semialdehyde dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BW25113(DE3) cells
-
gene davD, recombinant expression in Escherichia coli strain W3110, coexpression with Pseudomonas putida gene gabT, encoding 5-aminovalerate aminotransferase, and davA and davB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively. The recombinant Escherichia coli WL3110 strain expressingthe davAB and gabTD genes is cultured in a medium containing D-glucose, L-lysine, and 2-oxoglutarate, and produces glutarate
into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3)
-